<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>Nicole L. Jensen</dc:contributor>
  <dc:contributor>Katherine H. Haman</dc:contributor>
  <dc:contributor>William N. Batts</dc:contributor>
  <dc:contributor>Keith R. Jerome</dc:contributor>
  <dc:contributor>James Winton</dc:contributor>
  <dc:contributor>Alexander L. Greninger</dc:contributor>
  <dc:creator>Negar Makhsous</dc:creator>
  <dc:date>2017</dc:date>
  <dc:description>&lt;div id="ASec1" class="AbstractSection"&gt;&lt;p class="Heading"&gt;&lt;strong&gt;Background&lt;/strong&gt;&lt;/p&gt;&lt;p id="Par1" class="Para"&gt;Salmon are paramount to the economy, ecology, and history of the Pacific Northwest. Viruses constitute one of the major threats to salmon health and well-being, with more than twenty known virus species that infect salmon. Here, we describe the isolation and characterization of the fall Chinook aquareovirus, a divergent member of the species&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i class="EmphasisTypeItalic"&gt;Aquareovirus B&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;within the family&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i class="EmphasisTypeItalic"&gt;Reoviridae&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;&lt;div id="ASec2" class="AbstractSection"&gt;&lt;p class="Heading"&gt;&lt;strong&gt;Methods&lt;/strong&gt;&lt;/p&gt;&lt;p id="Par2" class="Para"&gt;The virus was first found in 2014 as part of a routine adult broodstock screening program in which kidney and spleen tissue samples from healthy-appearing, adult fall Chinook salmon (&lt;i class="EmphasisTypeItalic"&gt;Oncorhynchus tshawytscha&lt;/i&gt;) returning to a hatchery in Washington State produced cytopathic effects when inoculated onto a Chinook salmon embryo cell line (CHSE-214). The virus was not able to be confirmed by an RT-PCR assay using existing aquareovirus pan-species primers, and instead was identified by metagenomic next-generation sequencing. Metagenomic next-generation sequencing was used to recover the full genome and completed using 3′ RACE.&lt;/p&gt;&lt;/div&gt;&lt;div id="ASec3" class="AbstractSection"&gt;&lt;p class="Heading"&gt;&lt;strong&gt;Results&lt;/strong&gt;&lt;/p&gt;&lt;p id="Par3" class="Para"&gt;The genome of the fall Chinook aquareovirus contains 11 segments of double-stranded RNA totaling 23.3&amp;nbsp;kb, with each segment flanked by the canonical sequence termini found in the aquareoviruses. Sequence comparisons and a phylogenetic analysis revealed a nucleotide identity of 63.2% in the VP7 gene with the Green River Chinook virus, placing the new isolate in the species&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i class="EmphasisTypeItalic"&gt;Aquareovirus B&lt;/i&gt;. A qRT-PCR assay was developed targeting the VP2, which showed rapid growth of the isolate during the initial 5&amp;nbsp;days in culture using CHSE-214 cells.&lt;/p&gt;&lt;/div&gt;&lt;div id="ASec4" class="AbstractSection"&gt;&lt;p class="Heading"&gt;&lt;strong&gt;Conclusions&lt;/strong&gt;&lt;/p&gt;&lt;p id="Par4" class="Para"&gt;This sequence represents the first complete genome of an Aquareovirus B species. Future studies will be required to understand the potential pathogenicity and epidemiology of the fall Chinook aquareovirus.&lt;/p&gt;&lt;/div&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1186/s12985-017-0839-9</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>BioMed</dc:publisher>
  <dc:title>Isolation and characterization of the fall Chinook aquareovirus</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>