<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>Justin A. Mapula</dc:contributor>
  <dc:contributor>Scott A. Bonar</dc:contributor>
  <dc:creator>Olin Feuerbacher</dc:creator>
  <dc:date>2015</dc:date>
  <dc:description>&lt;p&gt;&lt;span&gt;Recent censuses of Devils Hole Pupfish&amp;nbsp;&lt;/span&gt;&lt;i&gt;Cyprinodon diabolis&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;revealed that fewer than 100 individuals currently remain in the wild. Captive propagation is among actions being considered to prevent their extinction, but no pure-strain Devils Hole Pupfish were available for broodstock. To help provide emergency information, we investigated techniques to propagate their most closely related relative, hybrid Devils Hole Pupfish&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;i&gt;C. diabolis&lt;/i&gt;&lt;span&gt;× Ash Meadows Amargosa Pupfish&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;i&gt;C. nevadensis mionectes.&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;We tested various temperatures and larval feeds with respect to egg production, larval survival, and growth. Larval survival and growth were similar from 24°C to 32°C and egg production peaked at static 28°C; however, reducing water temperatures to 23°C and then raising them to 28°C resulted in even higher production. Larvae fed infusoria, Rio Grande Silvery Minnow Chow (RGSM), or Zeigler larval diet (ZLD) had the highest survival (79.4, 71.6, and 73.4%, respectively), and those fed Otohime (OTO) had the lowest survival (60.8%), although OTO provided greatest (14&amp;nbsp;mm) 30-d growth. Supplementation of RGSM or ZLD with&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;i&gt;Artemia&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;nauplii increased growth but decreased survival. Larval production was maximized by placing six spawning mops, constructed of yarn and tile, in each of four 437-L parental aquaria, stocked with 24 adult fish each (1:1 sex ratio) for 3 d, to attract adults and provide spawning substrate. A 30% water change conducted on the same day of mop placement lowered water temperature from 28°C to 23°C. Water temperature was raised back to 28°C over 48&amp;nbsp;h. After 3 d, mops were transferred to hatching aquaria that were held at 28°C and aerated until larval hatch. Although some differences likely exist in effective propagation techniques for hybrid and pure-strain Devils Hole Pupfish, these data help provide initial recommendations to aid recovery.&lt;/span&gt;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1080/15222055.2015.1044057</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Taylor &amp; Francis</dc:publisher>
  <dc:title>Propagation of hybrid Devils Hole Pupfish × Ash Meadows Amargosa Pupfish</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>