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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>Susan Yun</dc:contributor>
  <dc:contributor>J. Lewis</dc:contributor>
  <dc:contributor>Michael T. Kearney</dc:contributor>
  <dc:contributor>John D. Hansen</dc:contributor>
  <dc:creator>Esteban Soto</dc:creator>
  <dc:date>2017</dc:date>
  <dc:description>&lt;p&gt;&lt;i&gt;Francisella noatunensis&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;subsp.&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;i&gt;orientalis&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;(&lt;/span&gt;&lt;i&gt;Fno&lt;/i&gt;&lt;span&gt;) (syn.&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;i&gt;F.&amp;nbsp;asiatica&lt;/i&gt;&lt;span&gt;) is an emergent warmwater fish pathogen and the causative agent of piscine francisellosis. Although&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;i&gt;Fno&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;causes septicemia and can live extracellularly in infected tilapia (&lt;/span&gt;&lt;i&gt;Oreochromis&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;spp.), the early interaction of&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;i&gt;Fno&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;with vasculature endothelium is unknown. In the present study, we examined the interaction of wild-type&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;i&gt;Fno&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;(WT) and two&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;i&gt;Fno&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;knockout [intracellular growth loci C (Δ&lt;/span&gt;&lt;i&gt;iglC&lt;/i&gt;&lt;span&gt;) and pathogenicity determinant protein A (Δ&lt;/span&gt;&lt;i&gt;pdpA&lt;/i&gt;&lt;span&gt;)] strains with the endothelial&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;i&gt;O.&amp;nbsp;mossambicus&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;bulbus arteriosus cell line (TmB) at 25&amp;nbsp;°C and 30&amp;nbsp;°C. Similar amounts of WT, Δ&lt;/span&gt;&lt;i&gt;iglC,&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;and Δ&lt;/span&gt;&lt;i&gt;pdpA&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;attached and were detected intracellularly after 5&amp;nbsp;h of incubation at both temperatures; however temperature affected attachment and uptake. While significantly greater amounts of&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;i&gt;Fno&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;(WT, Δ&lt;/span&gt;&lt;i&gt;iglC,&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;and Δ&lt;/span&gt;&lt;i&gt;pdpA&lt;/i&gt;&lt;span&gt;) were detected intracellularly when TmB cells were incubated at 30&amp;nbsp;°C, bacteria attached to TmBs at greater levels at 25&amp;nbsp;°C. Only WT&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;i&gt;Fno&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;was able to replicate intracellularly at 25&amp;nbsp;°C, which resulted in&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;i&gt;Fno&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;mediated cytotoxicity and apoptosis at 24 and 72&amp;nbsp;h post-infection. WT&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;i&gt;Fno&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;incubated at 30&amp;nbsp;°C as well as Δ&lt;/span&gt;&lt;i&gt;iglC,&lt;/i&gt;&lt;span&gt;and Δ&lt;/span&gt;&lt;i&gt;pdpA&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;incubated at 25&amp;nbsp;°C and 30&amp;nbsp;°C were all defective for survival, replication, and the ability to cause cytotoxicity in TmB. Taken together, these results demonstrate that temperature plays a vital role for&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;i&gt;Fno&lt;/i&gt;&lt;span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;intracellular survival, persistence and cytotoxicity.&lt;/span&gt;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1016/j.micpath.2017.03.003</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Elsevier</dc:publisher>
  <dc:title>Interaction of Francisella noatunensis subsp. orientalis with Oreochromis mossambicus bulbus arteriosus cell line</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>