James N. Huckins Randal C. Clark John M. Besser 1994 <p><span>We have assessed a fractionation scheme for selenium species that separates Se-containing amino acids and other organoselenium compounds in aqueous samples. We investigated the retention of standard solutions of selenate (Se</span>⁺⁶<span>), selenite (Se</span>⁺⁴<span>), and selenomethionine (Se</span>⁻²<span>) by fractionation media (Sephadex A-25 ion-exchange resin, copper-treated Chelex-100 ligand-exchange resin, and activated charcoal) and by several types of membrane filters. The fractionation method successfully isolated Se from the standard solutions into appropriate fractions for radiometric quantitation of<span>&nbsp;</span></span>⁷⁵<span>Se. However, some filter media retained unacceptably large amounts of selenate and selenite. Mass balance microcosms were inoculated with green algae (</span><i><span id="MathJax-Element-3-Frame" class="MathJax_SVG" data-mathml="<math class=&quot;math&quot; xmlns=&quot;http://www.w3.org/1998/Math/MathML&quot;><mtext>Chlamydomonas</mtext></math>"><span class="MJX_Assistive_MathML">Chlamydomonas</span></span><span id="MathJax-Element-4-Frame" class="MathJax_SVG" data-mathml="<math class=&quot;math&quot; xmlns=&quot;http://www.w3.org/1998/Math/MathML&quot;><mtext>reinhardtii</mtext></math>"><span class="MJX_Assistive_MathML">reinhardtii</span></span></i><span>) previously exposed to inorganic<span>&nbsp;</span></span>⁷⁵<span>Se, and the fractionation scheme was used to examine the release of<span>&nbsp;</span></span>⁷⁵<span>Se species into water and air. The results of the microcosm exposure indicate that seasonal blooms and crashes of phytoplankton populations may produce increased concentrations of organoselenium species.</span></p> application/pdf 10.1016/0045-6535(94)90045-0 en Elsevier Separation of selenium species released from Se-exposed algae article