David M. Hayes
John L. Harris
Nathan A. Johnson
Cheryl L. Morrison
Michael S. Eackles
Tim King
Jess W. Jones
Eric M. Hallerman
Alan D. Christian
Charles R. Randklev
Kentaro Inoue
2018
<p><span>The Pleurobemini (Bivalvia: Unionida) represent approximately one-third of freshwater mussel diversity in North America. Species identification within this group is challenging due to morphological convergence and phenotypic plasticity. Accurate species identification, including characterisation of currently unrecognised taxa, is required to develop effective conservation strategies because many species in the group are imperiled. We examined 575 </span><i>cox1</i><span><span> </span>sequences from 110 currently recognised species (including 13<span> </span></span><i>Fusconaia</i><span><span> </span>and 21<span> </span></span><i>Pleurobema</i><span><span> </span>species) to understand phylogenetic relationships among pleurobemine species (mainly<span> </span></span><i>Fusconaia</i><span><span> </span>and<span> </span></span><i>Pleurobema</i><span>) and to delineate species boundaries. The results of phylogenetic analyses showed no geographic structure within widespread species and illustrated a close relationship between<span> </span></span><i>Elliptio lanceolata</i><span><span> </span>and<span> </span></span><i>Parvaspina collina</i><span>. Constraint tests supported monophyly of the genera<span> </span></span><i>Fusconaia</i><span><span> </span>and<span> </span></span><i>Pleurobema</i><span>, including the subgenus<span> </span></span><i>P</i><span>. (</span><i>Sintoxia</i><span>). Furthermore, results revealed multiple conspecifics, including<span> </span></span><i>P. hanleyianum</i><span><span> </span>and<span> </span></span><i>P. troschelianum</i><span>,<span> </span></span><i>P. chattanoogaense</i><span><span> </span>and<span> </span></span><i>P. decisum</i><span>,<span> </span></span><i>P. clava</i><span><span> </span>and<span> </span></span><i>P. oviforme</i><span>,<span> </span></span><i>P. rubrum</i><span><span> </span>and<span> </span></span><i>P. sintoxia</i><span>,<span> </span></span><i>F. askewi</i><span><span> </span>and<span> </span></span><i>F. lananensis</i><span>, and<span> </span></span><i>F. cerina</i><span><span> </span>and<span> </span></span><i>F. flava</i><span>. Species delimitation analyses identified three currently unrecognised taxa (two in<span> </span></span><i>Fusconaia</i><span><span> </span>and one in<span> </span></span><i>Pleurobema</i><span>). Further investigation using additional genetic markers and other lines of evidence (e.g. morphology, life history, ecology) are necessary before any taxonomic changes are formalised.</span></p>
application/pdf
10.1071/IS17059
en
CSIRO Publishing
The Pleurobemini (Bivalvia: Unionida) revisited: Molecular species delineation using a mitochondrial DNA gene reveals multiple conspecifics and undescribed species
article