Deborah D. Iwanowicz
Cynthia R. Adams
Teresa Lewis
Tom Brandt
Lakyn R. Sanders
Robert S. Cornman
Luke R. Iwanowicz
2019
<p class="abstract_block">The fountain darter<span> </span><i>Etheostoma fonticola</i><span> </span>(FOD) is a federally endangered fish listed under the US Endangered Species Act. Here, we identified and characterized a novel aquareovirus isolated from wild fountain darters inhabiting the San Marcos River. This virus was propagated in Chinook salmon embryo (CHSE)-214, rainbow trout gonad-2 and fathead minnow cells at 15°C. The epithelioma papulosum cyprini cell line was refractory at all temperatures evaluated. High throughput sequencing technologies facilitated the complete genome sequencing of this virus utilizing ribosomal RNA-depleted RNA extracted from infected CHSE-214 cells. Conventional PCR primer sets were developed for the detection and confirmation of this virus to assist diagnostic screening methods. Phylogenetic analysis suggests this virus belongs to the<span> </span><i>Aquareovirus A</i><span> </span>genus. This research provides requisite initial data critical to support hatchery and refugia biosecurity measures for this endangered species.</p>
application/pdf
10.3354/dao03261
en
Inter-Research
Isolation, characterization and molecular identification of a novel aquareovirus that infects the endangered fountain darter, Etheostoma fonticola
article