J. Teska
1994
<p><span>Laboratory and field trials were conducted to evaluate in vitro growth of </span><i>Renibacterium salmoninarum</i><span> in media without serum or charcoal. Growth of this bacterium, the cause of bacterial kidney disease (BKD) in salmonids, is accelerated by addition of a growth enhancing “metabolite” of unknown composition to KDM2 medium, the medium commonly used for isolation of </span><i>R. salmoninarum</i><span>. KDM2 medium supplemented with greater than 1% (v/v) metabolite enhanced growth even without addition of either serum or charcoal. Medium containing 5% metabolite (denoted Five-M) allowed optimal growth in laboratory studies and was further evaluated as a primary plating medium for recovery of the bacterium isolated from chinook salmon (</span><i>Oncorhynchus tshawytscha</i><span>) exhibiting clinical BKD. Recovery rates of </span><i>R. salmoninarum</i><span> using Five-M medium were 4% and 36% higher, respectively, than comparable rates using a serum-based medium for the two salmon populations evaluated. Five-M medium is an effective, inexpensive alternative to serum-based or charcoal-based media.</span></p>
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10.7589/0090-3558-30.3.383
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Wildlife Disease Association
In vitro growth of the bacterial kidney disease organism Renibacterium salmoninarum on a nonserum, noncharcoal-based "homospecies-metabolite" medium
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