<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>Jeffrey M. Lorch</dc:contributor>
  <dc:creator>David S. Blehert</dc:creator>
  <dc:date>2021</dc:date>
  <dc:description>&lt;p&gt;&lt;i&gt;Pseudogymnoascus destructans&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;is a fungal pathogen that causes white‐nose syndrome, an emerging and fatal disease of North American bats that has led to unprecedented population declines. As a psychrophile,&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;P. destructans&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;is adapted to infect bats during winter hibernation, when host metabolic activity and core body temperature are greatly reduced. The ability to maintain and cultivate isolates of&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;P. destructans&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;in the laboratory is necessary for conducting research with this fungus. This article describes protocols for culturing&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;P. destructans&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;from bat wing skin and soil, for cryopreserving the fungus, and for preparing liquid suspensions for laboratory experimentation.&amp;nbsp;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1002/cpz1.23</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Wiley</dc:publisher>
  <dc:title>Laboratory maintenance and culture of Pseudogymnoascus destructans, the fungus that causes bat white-nose syndrome</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>