Ciaran A. Shaughnessy
Stephen D. McCormick
Andre Barany-Ruiz
2021
<p><span>Anadromous sea lamprey (</span><i>Petromyzon marinus</i><span>) larvae undergo a months-long true metamorphosis during which they develop seawater (SW) tolerance prior to downstream migration and SW entry. We have previously shown that intestinal Na</span><sup>+</sup><span>/K</span><sup>+</sup><span>-ATPase (NKA) activity increases during metamorphosis and is critical to the osmoregulatory function of the intestine in SW. The present study investigated the role of 11-deoxycortisol (S) in controlling NKA in the anterior (AI) and posterior (PI) intestine during sea lamprey metamorphosis. In a tissue profile, </span><i>nka</i><span> mRNA and protein were most abundant in the gill, kidney, and AI. During metamorphosis, AI </span><i>nka</i><span> mRNA increased 10-fold, whereas PI </span><i>nka</i><span> mRNA did not change. Specific corticosteroid receptors were found in the AI, which had a higher binding affinity for S compared to 11-deoxycorticosterone (DOC). </span><i>In vivo</i><span> administration of S in mid-metamorphic lamprey upregulated NKA activity 3-fold in the AI and PI, whereas administration of DOC did not affect intestinal NKA activity. During a 24 h SW challenge test, dehydration of white muscle moisture was rescued by prior treatment with S, which was associated with increased intestinal </span><i>nka</i><span> mRNA and NKA activity. These results indicate that intestinal osmoregulation in sea lamprey is a target for control by S during metamorphosis and the development of SW tolerance.</span></p>
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10.1016/j.ygcen.2021.113756
en
Elsevier
Corticosteroid control of Na+/K+-ATPase in the intestine of the sea lamprey (Petromyzon marinus)
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