Cheyenne R. Smith
Vicki S. Blazer
Luke R. Iwanowicz
Mary Ann Vogelbein
Stephen Kaattari
Christopher Ottinger
2021
<p><span>A murine monoclonal antibody (mAb, IgG2a) was produced for the detection of smallmouth bass (</span><span><i>Micropterus dolomieu</i></span><span>) immunoglobulin (IgM). The antibody is specific for IgM heavy chain and was shown to also recognize the Ig heavy chain of largemouth bass (</span><i>Micropterus salmoides</i><span>) and bluegill (</span><i>Lepomis macrochirus</i><span>) using Western Blot analysis of plasma from 9 teleost taxa. When applied to the analysis of smallmouth bass total plasma IgM using ELISA, the mAb was found to be effective when used in an inhibition kinetic assay.</span></p>
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10.1016/j.fsi.2021.03.006
en
Elsevier
Production and characterization of a mouse monoclonal antibody against smallmouth bass (Micropterus dolomieu) IgM
article