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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>John M. Sutton</dc:contributor>
  <dc:contributor>Janna L. Fierst</dc:contributor>
  <dc:contributor>Denise M. Akob</dc:contributor>
  <dc:contributor>Ronald S. Oremland</dc:contributor>
  <dc:creator>Shaun Baesman</dc:creator>
  <dc:date>2021</dc:date>
  <dc:description>&lt;p&gt;A Gram-stain-negative, strictly anaerobic, non-motile, rod-shaped bacterium, designated SFB93&lt;sup&gt;&lt;span class="jp-sup"&gt;T&lt;/span&gt;&lt;/sup&gt;, was isolated from the intertidal sediments of South San Francisco Bay, located near Palo Alto, CA, USA. SFB93&lt;sup&gt;&lt;span class="jp-sup"&gt;T&lt;/span&gt;&lt;/sup&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;was capable of acetylenotrophic and diazotrophic growth, grew at 22–37 °C, pH 6.3–8.5 and in the presence of 10–45 g l&lt;sup&gt;&lt;span class="jp-sup"&gt;−1&lt;/span&gt;&lt;/sup&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;NaCl. Phylogenetic analyses based on 16S rRNA gene sequencing showed that SFB93&lt;sup&gt;&lt;span class="jp-sup"&gt;T&lt;/span&gt;&lt;/sup&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;represented a member of the genus&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;span class="jp-italic"&gt;&lt;span class="named-content-genus"&gt;&lt;i&gt;Syntrophotalea&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;with highest 16S rRNA gene sequence similarities to&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;span class="jp-italic"&gt;&lt;span class="named-content-species"&gt;&lt;i&gt;Syntrophotalea acetylenica&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;DSM 3246&lt;sup&gt;&lt;span class="jp-sup"&gt;T&lt;/span&gt;&lt;/sup&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;(96.6 %),&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;span class="jp-italic"&gt;&lt;span class="named-content-species"&gt;&lt;i&gt;Syntrophotalea carbinolica&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;DSM 2380&lt;sup&gt;&lt;span class="jp-sup"&gt;T&lt;/span&gt;&lt;/sup&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;(96.5 %), and&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;span class="jp-italic"&gt;&lt;span class="named-content-species"&gt;&lt;i&gt;Syntrophotalea venetiana&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;DSM 2394&lt;sup&gt;&lt;span class="jp-sup"&gt;T&lt;/span&gt;&lt;/sup&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;(96.7 %). Genome sequencing revealed a genome size of 3.22 Mbp and a DNA G+C content of 53.4 %. SFB93&lt;sup&gt;&lt;span class="jp-sup"&gt;T&lt;/span&gt;&lt;/sup&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;had low genome-wide average nucleotide identity (81–87.5 %) and &amp;lt;70 % digital DNA–DNA hybridization value with other members of the genus&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;span class="jp-italic"&gt;&lt;span class="named-content-genus"&gt;&lt;i&gt;Syntrophotalea&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;. The phylogenetic position of SFB93&lt;span class="jp-sup"&gt;T&lt;/span&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;within the family&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;span class="jp-italic"&gt;&lt;span class="named-content-family"&gt;&lt;i&gt;Syntrophotaleaceae&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;and as a novel member of the genus&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;span class="jp-italic"&gt;&lt;span class="named-content-genus"&gt;&lt;i&gt;Syntrophotalea&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;was confirmed via phylogenetic reconstruction based on concatenated alignments of 92 bacterial core genes. On the basis of the results of phenotypic, genotypic and phylogenetic analyses, a novel species,&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;&lt;span class="jp-italic"&gt;Syntrophotalea acetylenivorans&lt;/span&gt;&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;sp. nov., is proposed, with SFB93&lt;sup&gt;&lt;span class="jp-sup"&gt;T&lt;/span&gt;&lt;/sup&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;(=DSM 106009&lt;sup&gt;&lt;span class="jp-sup"&gt;T&lt;/span&gt;&lt;/sup&gt;=JCM 33327&lt;sup&gt;&lt;span class="jp-sup"&gt;T&lt;/span&gt;&lt;/sup&gt;=ATCC TSD-118&lt;sup&gt;&lt;span class="jp-sup"&gt;T&lt;/span&gt;&lt;/sup&gt;) as the type strain.&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1099/ijsem.0.004698</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Microbiology Society</dc:publisher>
  <dc:title>Syntrophotalea acetylenivorans sp. nov., a diazotrophic, acetylenotrophic anaerobe isolated from intertidal sediments</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>