<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>Vicki S. Blazer</dc:contributor>
  <dc:contributor>Patricia M. Mazik</dc:contributor>
  <dc:creator>Heather L. Walsh</dc:creator>
  <dc:date>2022</dc:date>
  <dc:description>&lt;p&gt;&lt;span&gt;Histopathological assessments of young-of-the-year (age-0) Smallmouth Bass&amp;nbsp;&lt;/span&gt;&lt;i&gt;Micropterus dolomieu&lt;/i&gt;&lt;span&gt;&amp;nbsp;in the Susquehanna River drainage identified a high prevalence of the myxozoan&amp;nbsp;&lt;/span&gt;&lt;i&gt;Myxobolus inornatus&lt;/i&gt;&lt;span&gt;. This myxozoan infects the connective tissue of the muscle below the skin but is sometimes observed in the esophagus and buccal cavity. In some instances, shallow infections cause breaks in the skin, which could increase the chance of opportunistic bacterial infections. Several microbial pathogens, including&amp;nbsp;&lt;/span&gt;&lt;i&gt;Flavobacterium columnare&lt;/i&gt;&lt;span&gt;,&amp;nbsp;&lt;/span&gt;&lt;i&gt;Aeromonas&lt;/i&gt;&lt;span&gt;&amp;nbsp;spp., and Largemouth Bass virus, have also been cultured from clinically diseased young of year. A multiplex fluorescence in&amp;nbsp;situ hybridization (FISH) assay was developed to determine potential colocalization of&amp;nbsp;&lt;/span&gt;&lt;i&gt;M. inornatus&lt;/i&gt;&lt;span&gt;,&amp;nbsp;&lt;/span&gt;&lt;i&gt;Flavobacterium&lt;/i&gt;&lt;span&gt;&amp;nbsp;spp., and&amp;nbsp;&lt;/span&gt;&lt;i&gt;Aeromonas&lt;/i&gt;&lt;span&gt;&amp;nbsp;spp. infections. With FISH, 75% of age-0 Smallmouth Bass exhibited&amp;nbsp;&lt;/span&gt;&lt;i&gt;M. inornatus&lt;/i&gt;&lt;span&gt;&amp;nbsp;infections, 10% had&amp;nbsp;&lt;/span&gt;&lt;i&gt;Aeromonas&lt;/i&gt;&lt;span&gt;&amp;nbsp;spp. infections, and 5% had&amp;nbsp;&lt;/span&gt;&lt;i&gt;Flavobacterium&lt;/i&gt;&lt;span&gt;&amp;nbsp;spp. infections, while 3% had coinfections with both bacterial species and&amp;nbsp;&lt;/span&gt;&lt;i&gt;M. inornatus&lt;/i&gt;&lt;span&gt;. The results of the multiplex FISH assay revealed a low occurrence of coinfections of&amp;nbsp;&lt;/span&gt;&lt;i&gt;Flavobacterium&lt;/i&gt;&lt;span&gt;&amp;nbsp;spp. and/or&amp;nbsp;&lt;/span&gt;&lt;i&gt;Aeromonas&lt;/i&gt;&lt;span&gt;&amp;nbsp;spp. with&amp;nbsp;&lt;/span&gt;&lt;i&gt;M. inornatus&lt;/i&gt;&lt;span&gt;&amp;nbsp;in randomly sampled individuals.&lt;/span&gt;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1002/aah.10144</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>American Fisheries Society</dc:publisher>
  <dc:title>Development of a multiplex fluorescence in situ hybridization assay to identify coinfections in young-of-the-year smallmouth bass</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>