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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>Denise M. Akob</dc:contributor>
  <dc:contributor>Michelle M. Lorah</dc:contributor>
  <dc:contributor>Thomas E. Imbrigiotta</dc:contributor>
  <dc:contributor>Ronald W. Harvey</dc:contributor>
  <dc:contributor>Claire R. Tiedeman</dc:contributor>
  <dc:creator>Jennifer C. Underwood</dc:creator>
  <dc:date>2022</dc:date>
  <dc:description>&lt;p class="chapter-para"&gt;Bioaugmentation is a promising strategy for enhancing trichloroethylene (TCE) degradation in fractured rock. However, slow or incomplete biodegradation can lead to stalling at degradation byproducts such as 1,2-dichloroethene (&lt;i&gt;cis&lt;/i&gt;-DCE) and vinyl chloride (VC). Over the course of 7 years, we examined the response of groundwater microbial populations in a bioaugmentation test where an emulsified vegetable oil solution (EOS&lt;sup&gt;®&lt;/sup&gt;) and a dechlorinating consortium (KB-1&lt;sup&gt;®&lt;/sup&gt;), containing the established dechlorinator&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Dehalococcoides&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;(DHC), were injected into a TCE-contaminated fractured rock aquifer. Indigenous microbial communities responded within 2 days to added substrate and outcompeted KB-1&lt;sup&gt;®&lt;/sup&gt;, and over the years of monitoring, several other notable turnover events were observed. Concentrations of ethene, the end product in reductive dechlorination, had the strongest correlations (&lt;i&gt;P&lt;/i&gt;&amp;lt; .05) with members of Candidatus&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Colwellbacteria&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;but their involvement in reductive dechlorination is unknown and warrants further investigation.DHC never exceeded 0.6% relative abundance of groundwater microbial communities, despite its previously presumed importance at the site. Increased concentrations of carbon dioxide, acetic acid, and methane were positively correlated with increasing ethene concentrations; however, concentrations of&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;cis-&lt;/i&gt;DCE and VC remained high by the end of the monitoring period suggesting preferential enrichment of indigenous partial dechlorinators over bioaugmented complete dechlorinators. This study highlights the importance of characterizing&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;in situ&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;microbial populations to understand how they can potentially enhance or inhibit augmented TCE degradation.&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1093/femsec/fiac077</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Oxford University Press</dc:publisher>
  <dc:title>Microbial community response to a bioaugmentation test to degrade trichloroethylene in a fractured rock aquifer, Trenton, N.J</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>