Ryan Sweeney
David S. Blehert
Erica Spackman
David L. Suarez
Darrel Kapczynski
Kelsey Briggs
2023
<div id="abstracts" class="Abstracts u-font-gulliver text-s"><div id="abs0010" class="abstract author" lang="en"><div id="abssec0010"><p id="abspara0010">Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is believed to have a zoonotic origin with bats suspected as a natural host. In this work, we individually express the ACE2 of seven bat species including, little brown, great roundleaf, Pearson's horseshoe, greater horseshoe, Brazilian free-tailed, Egyptian rousette, and Chinese rufous horseshoe in DF1 cells and determine their ability to support attachment and replication of SARS-CoV-2 viruses. We demonstrate that the ACE2 receptor of all seven species made DF1 cells permissible to SARS-CoV-2. The level of virus replication differed between bat species and variants tested. The Wuhan lineage SARS-CoV-2 virus replicated to higher titers than either variant virus tested. All viruses tested grew to higher titers in cells expressing the human ACE2 gene compared to a bat ACE2. This study provides a practical<span> </span><i>in vitro</i>method for further testing of animal species for potential susceptibility to current and emerging SARS-CoV-2 viruses.</p></div></div></div>
application/pdf
10.1016/j.virol.2023.07.002
en
Elsevier
SARS-CoV-2 utilization of ACE2 from different bat species allows for virus entry and replication in vitro
article