<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>Carl O. Ostberg</dc:contributor>
  <dc:creator>Marshal S. Hoy</dc:creator>
  <dc:date>2023</dc:date>
  <dc:description>&lt;div id="Abs1-section" class="c-article-section"&gt;&lt;div id="Abs1-content" class="c-article-section__content"&gt;&lt;p&gt;We developed a primer and probe based quantitative PCR assay for use with environmental DNA to detect Northwest salamander (&lt;i&gt;Ambystoma gracile&lt;/i&gt;), a species endemic to the temperate Pacific coastal region of North America. The assay targets a region in the mitochondrial DNA (mtDNA) D-loop gene. Tests of the assay were performed&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;in silico&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;(using the NCBI BLAST tool), in vitro (using DNA extracted from&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;A. gracile&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;and related species) and in situ (water samples collected from a lake with a known population of&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;A. gracile&lt;/i&gt;). This assay will be useful in efforts to monitor the distribution and occupancy of this species.&lt;/p&gt;&lt;/div&gt;&lt;/div&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1007/s12686-023-01308-4</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Springer</dc:publisher>
  <dc:title>Development of a quantitative PCR assay for detecting northwest salamander (ambystoma gracile) in environmental DNA samples</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>