Brian R Krieser
Richard M. Pendleton
Barbara A. Lubinski
Robin L. Johnson
Dewayne A. Fox
Joel P Van Eenennaam
Jason E Kahn
Chris H Hager
Amanda L. Higgs
David C. Kazyak
Nicholas M Sard
2024
<div id="Abs1-section" class="c-article-section"><div id="Abs1-content" class="c-article-section__content"><p>Despite the importance of sex-specific information for sturgeon conservation and management, sex identification has been a major challenge outside of mature adults on spawning grounds. Recent work identified a sex-specific locus (<i>AllWSex2</i>) that appears to be broadly conserved across many Acipenserids, but the assay was not validated for all species within the family. We tested the<span> </span><i>AllWSex2</i><span> </span>marker in three sturgeon taxa (shortnose sturgeon<span> </span><i>Acipenser brevirostrum</i>, Gulf sturgeon<span> </span><i>A. oxyrhinchus desotoi</i>, and Atlantic sturgeon<span> </span><i>A. oxyrhinchus oxyrhinchus</i>) from the Atlantic and Gulf of Mexico Coasts of North America to validate its use for sex identification. Our results indicate<span> </span><i>AllWSex2</i><span> </span>is conserved in all three taxa, presenting a new opportunity to derive sex-specific information from tissue samples, which are routinely collected from these taxa. We found high concordance (range: 97–100%) between genotypic and phenotypic/histological methods, suggesting the assay is broadly effective. However, the small amount of discordance between the methods (< 3%) suggests further refinement may be possible.</p></div></div>
application/pdf
10.1007/s12686-024-01346-6
en
Springer
Validation of a molecular sex marker in three sturgeons from eastern North America
article