Jonathan Giersch Adam Sepulveda Clint C. Muhlfeld Patrick Ross Hutchins 2023 <div id="Abs1-section" class="c-article-section"><div id="Abs1-content" class="c-article-section__content"><p>A probe-based quantitative real-time PCR assay was developed to detect meltwater stonefly (<i>Lednia tumana</i>) environmental (e)DNA in water samples. The limits of detection and quantification, respectively, were 12.1 and 58.4 gene copies for calibration standards and these values were similarly low in a relevant environmental sample matrix (8.6 and 174.2, respectively). The assay’s utility was demonstrated in situ on water samples with concomitant manual invertebrate surveys from a wide range of alpine streams across<span>&nbsp;</span><i>L. tumana</i>’s native range.</p></div></div> application/pdf 10.1007/s12686-023-01307-5 en Springer Validation of a species-specific probe-based qPCR assay for the threatened meltwater stonefly, Lednia tumana, in environmental samples article