Petr Procházka Luana Bontempo Federica Camin Frederic Jiguet Gergely Osvath Craig A. Stricker Michael B. Wunder Rebecca L. Powell Vojtech Brlik 2024 <p><span>Geographic distribution models of environmentally stable isotopes (the so-called “isoscapes”) are widely employed in animal ecology, and wildlife forensics and conservation. However, the application of isoscapes is limited to elements and regions for which the spatial patterns have been estimated. Here, we focused on the ubiquitous yet less commonly used stable sulfur isotopes (δ</span>³⁴<span>S). To predict the European δ</span>³⁴<span>S isoscape, we used 242 feather samples from Eurasian Reed Warbler (</span><i>Acrocephalus scirpaceus</i><span>) formed at 69 European wetland sites. We quantified the relationships between sample δ</span>³⁴<span>S and environmental covariates using a random forest regression model and applied the model to predict the geographic distribution of δ</span>³⁴<span>S. We also quantified within-site variation in δ</span>³⁴<span>S and complementarity with other isotopes on both individual and isoscape levels. The predicted feather δ</span>³⁴<span>S isoscape shows only slight differences between the central and southern parts of Europe while the coastal regions were most enriched in&nbsp;</span>³⁴<span>S. The most important covariates of δ</span>³⁴<span>S were distance to coastline, surface elevation, and atmospheric concentrations of SO</span>₂<span>&nbsp;gases. The absence of a systematic spatial pattern impedes the application of the δ</span>³⁴<span>S isoscape, but high complementarity with other isoscapes advocates the combination of multiple isoscapes to increase the precision of animal tracing. Feather δ</span>³⁴<span>S compositions showed considerable within-site variation with highest values in inland parts of Europe, likely attributed to wetland anaerobic conditions and redox sensitivity of sulfur. The complex European geography and topography as well as using δ</span>³⁴<span>S samples from wetlands may contribute to the absence of a systematic spatial gradient of δ</span>³⁴<span>S values in Europe. We thus encourage future studies to focus on the geographic distribution of δ</span>³⁴<span>S using tissues from diverse taxa collected in various habitats over large land masses in the world (i.e., Africa, South America, or East Asia).</span></p> application/pdf 10.1002/ecs2.4690 en Ecological Society of America Geographic distribution of feather δ34S in Europe article