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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>Nathan D. Sy</dc:contributor>
  <dc:contributor>Philip Tanabe</dc:contributor>
  <dc:contributor>Chenyang Ji</dc:contributor>
  <dc:contributor>Jay Gan</dc:contributor>
  <dc:contributor>Daniel Schlenk</dc:contributor>
  <dc:creator>Jason Tyler Magnuson</dc:creator>
  <dc:date>2024</dc:date>
  <dc:description>&lt;div id="ab0005" class="abstract author" lang="en"&gt;&lt;div id="as0005"&gt;&lt;p id="sp0015"&gt;The frequency of detection and concentrations of bifenthrin, a pyrethroid insecticide, in the waterways inhabited by the endangered species, steelhead trout (&lt;i&gt;Oncorhynchus mykiss&lt;/i&gt;), has become a significant concern for regulatory agencies. Endocrine disruption has been observed with estrogenic and anti-estrogenic responses in fish species at different life stages. Since several studies have indicated alterations in dopaminergic signaling associated with endocrine responses, juvenile steelhead were exposed to environmentally relevant concentrations of 60 or 120&amp;nbsp;ng/L bifenthrin for two weeks. Fish brains were assessed for dopamine levels and the expression of genes involved in dopaminergic and estrogenic processes, such as catechol-&lt;i&gt;o&lt;/i&gt;-methyltransferase (&lt;i&gt;comt&lt;/i&gt;) and monoamine oxidase (&lt;i&gt;mao&lt;/i&gt;). Vitellogenin (&lt;i&gt;vtg&lt;/i&gt;) and estrogenic receptors (&lt;i&gt;ERα1&lt;/i&gt;,&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;ERβ1&lt;/i&gt;, and&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;ERβ2&lt;/i&gt;) were also evaluated in livers of the animals. Dopamine concentrations were significantly higher in fish brains following bifenthrin exposure. Consistent with a reduction in dopamine clearance, there was a significant decrease in the mRNA expression of&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;comt&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;with increased bifenthrin concentration. Hepatic expression of&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;ERα1&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;and&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;ERβ2&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;mRNA was significantly decreased with increased bifenthrin concentration. These data support the possible mechanism of bifenthrin altering the dopaminergic pathway at low ng/L concentrations, in juvenile steelhead, which could interfere with endocrine feedback loops. These findings support the need for and importance of identifying species and life stage differences in pesticide modes of action to reduce uncertainties in risk assessments.&lt;/p&gt;&lt;/div&gt;&lt;/div&gt;&lt;div id="ab0010" class="abstract graphical" lang="en"&gt;&lt;br&gt;&lt;/div&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1016/j.cbpc.2024.109995</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Elsevier</dc:publisher>
  <dc:title>Dopaminergic and anti-estrogenic responses in juvenile steelhead (Oncorhynchus mykiss) exposed to bifenthrin</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>