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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>Taniya RoyChowdhury</dc:contributor>
  <dc:contributor>Denise M. Akob</dc:contributor>
  <dc:contributor>Xuesong Zhang</dc:contributor>
  <dc:contributor>Gregory T. McCarty</dc:contributor>
  <dc:contributor>Stephanie A. Yarwood</dc:contributor>
  <dc:creator>Nora Hamovit</dc:creator>
  <dc:date>2025</dc:date>
  <dc:description>&lt;p&gt;&lt;span&gt;Wetlands are the largest natural source of methane (CH&lt;/span&gt;&lt;sub&gt;4&lt;/sub&gt;&lt;span&gt;), a potent greenhouse gas produced by methanogens. Methanogenesis rates are controlled by environmental factors such as redox potential, temperature, and carbon and electron acceptor availability and are presumably dependent on the composition of the active methanogen community. We collected intact soil cores from a restored and natural freshwater depressional wetland on Maryland’s Delmarva Peninsula (USA) to assess the effects of wetland restoration and redox shifts on microbial processes. Intact soil cores were incubated under either saturated (anoxic) or unsaturated (oxic) conditions and amended with&amp;nbsp;&lt;/span&gt;&lt;sup&gt;13&lt;/sup&gt;&lt;span&gt;C-acetate for quantitative stable isotope probing (qSIP) of the 16S rRNA gene. Restored wetland cores supported a distinct community of methanogens compared to natural cores, and acetoclastic methanogens putatively identified in the genus&amp;nbsp;&lt;/span&gt;&lt;i&gt;Methanosarcina&lt;/i&gt;&lt;span&gt;&amp;nbsp;were among the most abundant taxa in restored anoxic and oxic cores. The active microbial communities in the restored wetland cores were also distinguished by the unique presence of facultatively anaerobic bacteria belonging to the orders&amp;nbsp;&lt;/span&gt;&lt;i&gt;Firmicutes&lt;/i&gt;&lt;span&gt;&amp;nbsp;and&amp;nbsp;&lt;/span&gt;&lt;i&gt;Bacteroidetes&lt;/i&gt;&lt;span&gt;. In natural wetland incubations, methanogen populations were not among the most abundant taxa, and these communities were instead distinguished by the unique presence of aerobic bacteria in the phyla&amp;nbsp;&lt;/span&gt;&lt;i&gt;Acidobacteria&lt;/i&gt;&lt;span&gt;,&amp;nbsp;&lt;/span&gt;&lt;i&gt;Actinobacteria&lt;/i&gt;&lt;span&gt;, and class&amp;nbsp;&lt;/span&gt;&lt;i&gt;Alphaproteobacteria&lt;/i&gt;&lt;span&gt;. Iron-reducing bacteria, in the genus&amp;nbsp;&lt;/span&gt;&lt;i&gt;Geobacter&lt;/i&gt;&lt;span&gt;, were active across all redox conditions in both the restored and the natural cores, except the natural oxic–anoxic condition. These findings suggest an overall higher potential for methanogenesis in the restored wetland site compared to the natural wetland site, even when there is evidence of Fe reduction.&lt;/span&gt;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1128/aem.02161-24</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>American Society for Microbiology</dc:publisher>
  <dc:title>Comparative assessment of a restored and natural wetland using 13C-DNA SIP reveals a higher potential for methane production in the restored wetland</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>