<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>Umberto Rosani</dc:contributor>
  <dc:contributor>Arfa Khan</dc:contributor>
  <dc:contributor>Qiuwen Ding</dc:contributor>
  <dc:contributor>Eveline J. Emmenegger</dc:contributor>
  <dc:contributor>Zhuozhi Wang</dc:contributor>
  <dc:contributor>Thomas Nalpathamkalam</dc:contributor>
  <dc:contributor>Bhooma Thiruvahindrapuram</dc:contributor>
  <dc:creator>Sharon Clouthier</dc:creator>
  <dc:date>2025</dc:date>
  <dc:description>&lt;p&gt;&lt;span id="_mce_caret" data-mce-bogus="1" data-mce-type="format-caret"&gt;&lt;span&gt;DNA sequence from a new alloherpesvirus named acipenserid herpesvirus 3 (AciHV-3) was found in sturgeon species that are vulnerable to decline globally. A study was undertaken to develop a better understanding of the virus genome and to develop diagnostic tools to support an epidemiological investigation. A 184,426 bp genome was assembled from PacBio HiFi sequences generated with DNA from a Lake Sturgeon&amp;nbsp;&lt;/span&gt;&lt;span class="html-italic"&gt;Acipenser fulvescens&lt;/span&gt;&lt;span&gt;&amp;nbsp;gonad cell line. The AciHV-3 genome was contiguous with host chromosomal DNA and was structured with telomere-like terminal direct repeat regions, five internal direct repeat regions and a U region that included intact open reading frames encoding alloherpesvirus core proteins. Diagnostic testing conducted with a newly developed and analytically validated qPCR assay established the ubiquitous presence and high titer of AciHV-3 DNA in somatic and germline tissues from wild Lake Sturgeon in the Hudson Bay drainage basin. Phylogenetic reconstructions confirm that the monophyletic AciHV-3 lineage shares a common ancestor with AciHV-1 and that AciHV-3 taxa cluster according to their sturgeon host. The same genotype of AciHV-3 is found in disjunctive Lake Sturgeon populations within and among drainage basins. The results support the hypotheses that AciHV-3 has established latency through germline chromosomal integration, is vertically transmitted via a Mendelian pattern of inheritance, is evolving in a manner consistent with a replication competent virus and has co-evolved with its host reaching genetic fixation in Lake Sturgeon populations in central Canada.&lt;/span&gt;&lt;/span&gt;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.3390/v17040534</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>MDPI</dc:publisher>
  <dc:title>Genomic and epidemiological investigations reveal chromosomal integration of the acipenserid herpesvirus 3 genome in Lake Sturgeon &lt;i&gt;Acipenser fulvescens&lt;/i&gt;</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>