Stephanie Beeler Joseph W. Duris 2008 <p class="body">The U.S. Geological Survey, in cooperation with the Lenawee County Conservation District in Lenawee County, Mich., conducted a sampling effort over a single growing season (June to November 2007) to evaluate the microbiological water quality around a novel livestock reservoir wetland sub-irrigation system. Samples were collected and analyzed for fecal coliform bacteria, <i>Escherichia coli</i> (<i>E. coli</i>) bacteria, and six genes from pathogenic strains of <i>E. coli</i>.</p><p class="body">A total of 73 water-quality samples were collected on nine occasions from June to November 2007. These samples were collected within the surface water, shallow ground water, and the manure-treatment system near Bakerlads Farm near Clayton in Lenawee County, Mich. Fecal coliform bacteria concentrations ranged from 10 to 1.26 million colony forming units per 100 milliliters (CFU/100 mL). <i>E. coli</i> bacteria concentrations ranged from 8 to 540,000 CFU/100 mL. Data from the <i>E. coli</i> pathogen analysis showed that 73 percent of samples contained the <i>eaeA</i> gene, 1 percent of samples contained the <i>stx2</i> gene, 37 percent of samples contained the <i>stx1</i> gene, 21 percent of samples contained the <i>rfb</i>O157 gene, and 64 percent of samples contained the LTIIa gene.</p> application/pdf 10.3133/ofr20081025 en U.S. Geological Survey Fecal-indicator bacteria and <i>Escherichia coli</i> pathogen data collected near a novel sub-irrigation water-treatment system in Lenawee County, Michigan, June-November 2007 reports