USGS Publications Warehouse

Treatability study to evaluate bioremediation of trichloroethene at Site K, former Twin Cities Army Ammunition Plant, Arden Hills, Minnesota, 2020–22

Fri, 20 Feb 2026 18:18:35

Executive Summary

Chlorinated solvents, including trichloroethene (TCE) and other chlorinated volatile organic compounds (cVOCs), are widespread contaminants that can be treated by bioremediation approaches that enhance anaerobic reductive dechlorination. Reductive dechlorination can be enhanced either through the addition of an electron donor (biostimulation) or the addition of a known dechlorinating culture (bioaugmentation) along with an electron donor. Although bioremediation has been applied at many TCE- contaminated groundwater sites, application in source zones at sites where residual dense nonaqueous phase liquid (DNAPL) is present is more limited. In this study, laboratory and field treatability tests were completed to evaluate the potential application of anaerobic bioremediation for a shallow groundwater plume containing TCE in a perched alluvial aquifer at Site K, former Twin Cities Army Ammunition Plant, Arden Hills, Minnesota, which was on the National Priorities List as the New Brighton/Arden Hills Superfund site until 2019. In addition to the presence of residual DNAPL at the site, temporal variability in groundwater flow directions and input of oxygenated recharge were possible complicating factors for the application of enhanced anaerobic biodegradation in the shallow plume. The Site K plume extends beneath the footprint of Building 103, which was demolished in 2006, and soil excavations to a maximum depth of 6 feet (ft) below ground surface in 2014 were known to leave some deeper contaminated soil in place in the TCE source area. Groundwater treatment at the site, formalized as part of the 1997 Record of Decision, has been in operation since 1986 and consists of an extraction trench at the downgradient edge of the plume to collect groundwater, which is then pumped to an on- site air stripper. Groundwater concentrations in the plume have been relatively stable since treatment began, indicating a continued source of TCE in the aquifer. The desire for a destructive remedy that would enhance the removal of cVOCs in the aquifer at Site K and shorten the remediation timeframe led the U.S. Army to request that the U.S. Geological Survey conduct a groundwater treatability study to assess bioremediation. This report describes the U.S. Geological Survey bioremediation treatability study conducted during 2020–22, including pre- design site characterization to assist in formulating the bioremediation approach, laboratory experiments to support the design of the field pilot test, and implementation and 1-year performance monitoring results for the pilot test.

Pre- design site characterization included the collection of soil cores for cVOC analysis and lithologic descriptions and the re- installment of three wells to obtain hydrologic measurements and initial groundwater chemistry. Relatively flat head gradients were measured at the site, and substantial decreases in water- level elevations occurred from spring to summer (May–July 2021). Continuous water- level monitoring indicated a rapid response to precipitation. Groundwater flow velocities were consistently less than 0.5 foot per day, and the pilot bioremediation test was therefore designed with short lateral distances (about 5 ft) between injection and individual monitoring points. Soil analyses confirmed that high volatile organic compound contamination was left in place in the source area. The highest concentrations were near or in clay at the base of the perched aquifer. Concentrations of cVOCs measured in the replaced wells were consistent with historical data and had a maximum TCE concentration of 57,700 micrograms per liter (μg/L), indicative of nearby residual DNAPL based on the general rule of observed concentrations exceeding 1 percent of solubility. The primary TCE daughter product detected was 1,2- cis- dichloroethene (cisDCE), which indicated limited reductive dechlorination in the plume. Groundwater in both the source and downgradient areas was relatively reducing during the pre- design characterization, particularly in the source area where methane concentrations greater than 400 μg/L were measured.

Initial laboratory tests conducted using native aquifer microorganisms from the three replacement wells showed that anaerobic TCE biodegradation rates were low when biostimulated with the addition of sodium lactate as an electron donor, also known as a carbon donor, and resulted in the production of only cisDCE. Addition of a known dechlorinating culture, WBC- 2, however, resulted in rapid biodegradation and production of ethene, verifying complete reductive dechlorination of TCE. Microcosms constructed with aquifer soil collected from the site were used to evaluate other electron donors besides lactate to support reductive dechlorination by WBC- 2, including corn syrup as an alternative fast- release compound and whey, soy- based vegetable oil, and 3- D Microemulsion (Regenesis, San Clemente, California) as slow-release compounds. First- order rate constants for total organic chlorine removal in these WBC- 2 amended microcosms were greatest with either lactate or vegetable oil as the donor, ranging between 0.061 and 0.047 per day or corresponding half- lives of 11–15 days. Testing of commercial products in other WBC- 2- bioaugmented microcosms led to selection for the field pilot test of an emulsified vegetable oil product that also contained some sodium lactate as a fast- release donor. Delaying the addition of WBC- 2 relative to the donor in the microcosms resulted in the most rapid overall biodegradation rates.

The selected design for the pilot test utilized three separate test plots, each about 30-ft wide and 60-ft long: plots GS1 and GS2 in the source area of the plume and plot GS3 in the downgradient area of the plume near the excavation trench. Each test plot had one injection well, one monitoring well upgradient from the injection point, and 12 surrounding monitoring wells in a grid to capture variable groundwater flow directions. Donor injections, which included a bromide tracer, were completed in October 2021, immediately following baseline sampling, and the WBC- 2 culture was injected about 40 days later, between November 30 and December 2, 2021. Performance monitoring conducted until December 2022 included hydrologic measurements and analyses of cVOCs, redox- sensitive constituents, dissolved organic carbon, bromide, volatile fatty acids, compound- specific carbon isotopes, and microbial communities.

The biogeochemical data collected during the pilot tests in the three treatment plots showed that enhanced, complete reductive dechlorination of cVOCs in the groundwater was achieved in the GS1 and GS3 plots. In contrast, evidence of distribution of the injected amendments and subsequent biodegradation was limited in GS2, which was in an area of more heterogeneous soil lithology and low water table elevations. The molar composition of volatile organic compounds in the GS1 and GS3 plots was dominated by ethene in wells that were reached by the injected amendments by the end of the monitoring period. In the GS1 and GS3 plots, similar patterns were observed of cVOC concentrations decreasing to near detection levels, or below, at some wells sampled in July and October 2022, whereas ethene became dominant and indicated sustained complete reductive dechlorination. Baseline cVOC concentrations were more than a factor of 10 higher in the groundwater in the GS1 plot than in GS3, but no apparent inhibition of complete dechlorination occurred. As expected from the initial pre- design site data and the laboratory experiments, enhanced dissolution of residual DNAPL coupled to biodegradation was evident in the GS1 plot, where a marked increase in dichloroethene (DCE) above the initial baseline and upgradient TCE and DCE concentrations occurred. DCE concentrations subsequently declined where DNAPL dissolution was evident, concurrent with production of vinyl chloride and then predominantly ethene. Thus, overall biodegradation rates outpaced the DNAPL dissolution and desorption and DCE production in the source area. This success in complete degradation to predominantly ethene was achieved even in areas where the DCE concentrations reached a maximum of about 30,000 μg/L. Compound specific isotope analysis of carbon in TCE, cisDCE, trans- 1,2- dichloroethene, and vinyl chloride was conducted to provide another line of evidence of the occurrence and extent of anaerobic biodegradation. Along a flow path in each plot that was affected by the injected amendments, carbon isotopes in the TCE and daughter cVOCs in the groundwater became isotopically heavier, indicating biodegradation.

Enhanced biodegradation rates calculated from the field tests in GS1 and GS3 showed half- lives of 36.9–75.3 days for DCE degradation and 9.48–38.5 days for ethene production. Notably, these ethene production rates calculated from the field tests are consistent with the results of WBC- 2- bioaugmented microcosms amended with either lactate or vegetable oil, which had half- lives for total organic chlorine removal that ranged from 11 to 15 days. These rates indicated rapid enhanced biodegradation, which is promising for application of a full- scale bioremediation remedy. Ultimately, however, the mass of residual or sorbed TCE in the aquifer that remains accessible for dissolution and biodegradation would likely control the time required for a full- scale bioremediation effort to achieve performance goals for TCE and cisDCE specified in the Record of Decision for Site K.

The field pilot tests showed that the relatively low hydraulic head gradients and temporal changes in groundwater flow directions in the shallow aquifer would add complexity to a full- scale bioremediation effort. The radius of influence (ROI) at GS1 and GS3 (16.3 ft and 12.7 ft, respectively) were close to the design ROI of 15 ft. The estimated ROI at GS2 was about four times the design ROI, but may be less reliable at this location owing to groundwater flow direction. In addition, the low temperatures following WBC- 2 injection in late November to early December 2021, in combination with the low hydraulic head gradients, were probably major factors in the delay observed before the onset of enhanced biodegradation following injection of the culture. Additional test injections could be beneficial to optimize the timing of donor and culture injections with the variable temperatures and hydraulic head in the shallow aquifer.

Channel change and sediment transport in the Puyallup River watershed through 2022

Anderson, Scott — Tue, 31 Mar 2026 13:38:43

The Puyallup River drains a 990 square mile watershed in western Washington, with headwaters on the glacier-covered flanks of Mount Rainier. Major tributaries include the White, Carbon, and Mowich Rivers. In the levee-confined reaches of the lower watershed, loss of flood conveyance due to sand and gravel deposition has been a chronic issue. Over much of the 20th century, flood conveyance was maintained through sediment removal, but this practice ended in the late 1990s. Flood hazard management activities since the 1990s have primarily involved levee removal or setback projects. Assessments of 1984-2009 repeat cross sections suggested that sediment deposition rates were particularly high in reaches with recent levee setbacks. However, there have been no assessments of recent deposition rates since the 2009 surveys. There are also concerns that intensifying flood hydrology or increased sediment delivery from Mount Rainier may exacerbate deposition. However, assessment of those risks has been hindered by limited understanding of watershed-scale sediment delivery and routing, particularly for coarse sand and gravel.

The U.S. Geological Survey, in cooperation with Pierce County, initiated this study to improve understanding of sediment deposition in the lower Puyallup River watershed. This work is primarily based on differencing of multiple aerial lidar datasets collected during 2002–2022, supplemented by early 1990 photogrammetric elevation datasets, geomorphic assessments of streamgage data, historical topographic surveys from 1907, and previously collected sediment transport measurements. Analyses cover the Puyallup, Carbon, and Mowich Rivers, but do not include the White River.

During 2004–2020, repeat aerial lidar indicates that 1.3 ± 0.3 million yd3 of sediment accumulated in the lower 20 valley miles (VMs) of the Puyallup River, averaging 80,000 ± 20,000 cubic yards per year (yd3/yr). Deposition was observed during both 2004–11 and 2011–20 lidar differencing intervals. This continued a long-term depositional trend that extends back to at least 1977. From 2004 to 2011, deposition rates along the Soldiers Home levee setback reach, the only setback project downstream of VM 20 completed prior to 2011, were approximately four times higher than in adjacent unmodified reaches. From 2011 to 2020, two additional setback projects were completed; volumetric deposition rates over all three setback reaches were similar to adjacent unmodified reaches, suggesting elevated setback deposition in the 2004–11 interval may have been influenced by an extreme flood in November 2006. These levee setback projects increased the local cross-sectional area of the floodway, used as a rough proxy for relative flood conveyance, by 50 to 200 percent above 2004 conditions. If deposition continued at recent rates, cross-sectional area over the levee setback reaches would be reduced back to 2004 values by 2050-90.

Deposition also occurred over the lower six VMs of the Carbon River during 2004–20, though volumes (0.15 ± 0.09 million yd3) were an order of magnitude lower than along the Puyallup River. Relatively lower deposition rates in the Carbon River are most likely the combined result of modestly lower incoming sediment loads, modestly steeper channel slope, and the additional sediment transport capacity provided by two large non-glacial tributaries that enter the Carbon River near VM 5.

Upstream of the depositional reaches described above, 2002–22 sediment storage trends along the Puyallup, Carbon, and Mowich Rivers were predominately negative (net erosion) up to the Mount Rainier National Park boundary. Net erosion was the result of bank and bluff erosion exceeding deposition across wetted channel and bare gravel areas, as opposed to uniform vertical downcutting. Net erosion along these river valleys delivered 3.4 ± 0.6 million yd3 to the river system, equivalent to 190,000 ± 35,000 yd3/yr. Most of that volume was supplied by erosion of relatively low (4–10 ft) surfaces along the Puyallup and Mowich Rivers and tall (300 ft) glacial bluffs along the lower Carbon River. Substantial aggradation from 1984 to 2009 reported by Czuba and others (2010) along reaches of the Puyallup River (VM 19–22) where levee confinement has recently been removed was most likely an artifact of methodologic bias.

The Puyallup, Mowich, and Carbon Rivers drain five distinct glaciated watersheds on the flanks of Mount Rainier, four of which were assessed in this study. All four watersheds were impacted by an extreme November 2006 rainstorm. Between 2002 and 2008, debris flows occurred in all four headwater areas, collectively eroding at least 2.1 million yd3 of sediment. These debris flows formed distinct deposits one to two miles downstream of source areas, depositing 30-50 percent of the material eroded upstream. From 2008 to 2022, no headwater debris flows were observed and overall rates of geomorphic change in the headwaters were low. Rivers eroded into debris flow deposits emplaced over the 2002–08 interval, but re-deposited equivalent volumes of material within a half mile downstream.

Stage-discharge relations at five streamgages on upland rivers draining Mount Rainier show either net channel incision or dynamic variability with no long-term trend over the past 60–100 years. Observations of pervasive river valley erosion and stable or incising trends at long-term streamgages in the upper watershed do not support prior claims of widespread and accelerating aggradation of upland rivers draining Mount Rainier.

Erosion and deposition volumes estimated in this report were combined with sediment transport estimates from limited suspended sediment and bedload measurements, estimates of sub-glacial erosion rates, and sediment delivery from non-glacial tributaries to construct watershed-scale sediment budgets for the Puyallup River watershed. During 2004–20, the estimated sediment load entering the depositional lowlands was well balanced by estimated inputs from, in order of relative magnitude, subglacial erosion (33–60 percent of total sediment load), erosion along the major river valleys (25–45 percent), erosion in recently deglaciated headwater areas (7–17 percent) and non-glacial tributaries (3–9 percent). These results are specific to the study period and represent total sediment loads, most of which is fine material carried in suspension. The relative sourcing of sand and gravel may be different than implied by this sediment budget.

Downstream of VM 12, comparison of 1907 and 2009 channel surveys show net lowering of the channel thalweg of 4–12 ft. A long-term gage near VM 22 shows lowering of 4–5 ft through the 1960s. Lowering at both locations was inferred to be a channel response to the substantial straightening, and so steepening, of the river during major phases of levee construction through the early and mid-20th century.

Application of a simple empirical bedload-discharge power-law relation to an ensemble of model-estimated daily mean discharge records in the lower Puyallup River between 1977 and 2100 projects that annual bedload transport capacity in the lower Puyallup River will increase by 20–60 percent by the middle of the 21st century. Actual changes in bedload transport and deposition rates will depend on concurrent changes in sediment supply and local hydraulics governing deposition.

This report presents several key conclusions. First, the persistence and spatial patterns of sand and gravel deposition along the lower Puyallup River support prior claims that deposition is fundamentally caused by decreases in channel slope moving downstream. Given this underlying cause and the abundance of sand and gravel available to be transported downstream, deposition is likely to continue for the foreseeable future. Second, despite continued sediment deposition, recent levee setback projects in the lower Puyallup River will likely provide several decades of flood conveyance benefits relative to a no-action alternative. Third, while the rivers linking Mount Rainier to the Puget Sound lowlands have often been discussed as conduits that either pass or accumulate sediment from Mount Rainier, observations from 2002–22 show these river valleys acting as substantial sediment sources, delivering three times more sediment than recently deglaciated headwater areas on Mount Rainier. While the persistence and underlying cause of recent river valley erosion remain unknown, sediment storage dynamics along these river valleys are likely to be a major control on sand and gravel delivery to the lower watershed.