USGS Georgia Water Science Center
This report is available online in pdf format (1 MB): USGS SIR 2004-5004 ()
Peter G. Hartel, Elizabeth A. Frick, Adrienne L. Funk, Jennifer L. Hill, Jacob D. Summer, and M. Brian Gregory
U.S. Geological Survey Scientific Investigations Report 2004-5004; 10 pages and interactive map (Published 2004)
Factors affecting bacterial source tracking are important to understand because they affect the amount of sampling needed to describe fecal sources in a watershed adequately. The study area was a 76-kilometer reach of the Chattahoochee River and its tributaries in Metropolitan Atlanta, Georgia. Escherichia coli was isolated from water samples collected during baseflow and stormflow conditions from four mainstem and eight tributary sites; 262 isolates were ribotyped and assessed for their similarity. The vast majority of the E. coli ribotype patterns were unshared, whether the comparisons were between baseflow and stormflow conditions at one location, or between one location and another. The data suggest that either baseflow and stormflow conditions affected sharing of ribotype patterns, or that the sample size was too small to characterize the sharing adequately. Regardless, the results suggest that a large sampling of E. coli isolates is needed during various flow conditions from watersheds with complex land-use patterns for adequate bacterial source tracking.
Materials and Methods
Selection and identification of E. coli isolates from water
DNA extraction, quantification, and ribotyping of E. coli isolates
This report is available online in pdf format (1 MB): USGS SIR 2004-5004 ()To view the PDF document, you need the Adobe Acrobat® Reader installed on your computer. (A free copy of the Acrobat® Reader may be downloaded from Adobe Systems Incorporated.)
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