Assessment of Water Quality, Benthic Invertebrates, and Periphyton in the Threemile Creek Basin, Mobile, Alabama, 1999–2003
U.S. Geological Survey, Scientific Investigations Report 2004–5302
by Ann K. McPherson, Amy C. Gill, and Richard S. Moreland
Table of Contents
Abstract
Introduction
Purpose and Scope
Study Area
Previous Investigations
Acknowledgments
Watershed Characteristics of the Threemile Creek Basin
Land Use
Hydrology
Methods of Study
Water-Quality Sample Collection
Benthic Invertebrate and Periphyton Sample Collection
Water-Quality Data Analysis and Review
Quality-Control Methods and Results
Benthic Invertebrate and Periphyton Data Analysis and Review
Water-Quality Results and Discussion
Basic Water Chemistry
Major Ions
Instantaneous and Continuous Measurements of Water Properties
Nutrients
Nitrogen
Phosphorus
Suspended Chlorophyll a and b
Biochemical Oxygen Demand and Total Organic Carbon
Fecal-Indicator Bacteria
Organic Wastewater Compounds
Occurrence and Distribution of Wastewater Indicator Groups
Organic Wastewater Compounds as Endocrine Disruptors
Antioxidants
Prescription and Nonprescription Drugs
SteroidsSterols and Stanols
Fragrances
Detergent Metabolites
Antimicrobials
Plasticizers/Flame Retardants
Insect Repellent
Insecticides
Herbicides and Fungicides
Polycyclic Aromatic Hydrocarbons
Comparison of Organic Wastewater Compounds in the Threemile Creek Basin to Sites Nationwide
Benthic Invertebrates Results and Discussion
Periphyton Results and Discussion
Summary
Selected References
Figures
1 4. Maps showing:
- Locations of sampling sites in the Threemile Creek basin, Mobile County,
Alabama
- Locations of stormwater outfalls, wastewater-treatment plants, sampling
sites, and profile sites in the Threemile Creek basin, Mobile, Alabama
- Delineations of the Level III and Level IV Ecoregion classifications and
subbasin boundaries in the Threemile Creek basin, Mobile, Alabama
- Land-use characteristics in the Threemile Creek basin, Mobile, Alabama
5. Graph showing land use in the Threemile Creek basin, Mobile, Alabama
6. Piper trilinear diagram showing major ion composition of surface-water samples
collected at six sites in the Threemile Creek basin, Mobile, Alabama, 20002001
738. Graphs showing:
- Gage height, water temperature, specific conductance, dissolved oxygen,
turbidity, and discharge at U.S. Geological Survey streamgaging stations
(A) 02471013 (TM-1), (B) 0247101490 (TM-4), and (C) 02471016 (TM-5),
August 25September 4, 2001
- Number of days that continuous water-quality monitors recorded
concentrations of dissolved oxygen less than 3.0 milligrams per liter at
three sites on Threemile Creek, Mobile, Alabama, water years 20002003
- Distribution of total nitrogen, nitrite plus nitrate, total ammonia plus organic
nitrogen, and ammonia concentrations at sites in the Threemile Creek basin,
Mobile, Alabama
- Median concentrations of nitrite plus nitrate, ammonia, and total organic
nitrogen at sites in the Threemile Creek Basin, Mobile, Alabama
- Distribution of total phosphorus, dissolved phosphorus, and dissolved
orthophosphate at sites in the Threemile Creek basin, Mobile, Alabama
- Median concentrations of suspended phosphorus and dissolved phosphorus
at sites in the Threemile Creek basin, Mobile, Alabama
- Distribution of dissolved inorganic nitrogen and dissolved orthophosphate
ratios for sites in the Threemile Creek Basin, Mobile, Alabama
- Concentrations of (A) enterococci, (B) Escherichia coli, and (C) fecal coliform
concentrations in water samples collected at sites in the Threemile Creek
basin, Mobile, Alabama, 20002003
- Concentrations of (A) enterococci, (B) Escherichia coli, and (C) fecal coliform
concentrations at three sites on Threemile Creek, Mobile, Alabama,
December 1315, 2001
- Concentrations of (A) enterococci, (B) Escherichia coli, and (C) fecal coliform
concentrations at three sites on Threemile Creek, Mobile, Alabama,
June 2426, 2002
- Concentrations of (A) enterococci and (B) Escherichia coli concentrations
at three sites on Threemile Creek, Mobile, Alabama, February 2628, 2003
- Concentrations of (A) enterococci, (B) Escherichia coli, and (C) fecal coliform
concentrations at three sites on Threemile Creek, Mobile, Alabama,
September 1316, 2003
- Relation between Escherichia coli and fecal coliform concentrations measured
at sites in the Threemile Creek basin, Mobile, Alabama, 20002003
- Frequencies of detection for selected organic wastewater compounds in water
samples from the Threemile Creek basin, Mobile, Alabama, 20002003
- Summary of organic wastewater compounds detected per sample for (A) total
concentration (summation of 48 compounds) and (B) number of compounds
detected
- The 10 most frequently detected organic wastewater compounds at each site
in the Threemile Creek basin, Mobile, Alabama, 20002003
- The 10 most frequently detected organic wastewater compounds in the
Threemile Creek basin, Mobile, Alabama, 20002003
- Relation between total concentration of organic wastewater compounds and
total phosphorus, Threemile Creek basin, Mobile, Alabama, 20002003
- Summary of the types of organic wastewater compounds detected per sample
for (A) total concentration (summation of 48 compounds) and (B) number of
compounds detected, Threemile Creek basin, Mobile, Alabama, 20002003
- (A) Frequency of detection of organic wastewater compounds by general-use
category and (B) percentage of total measured concentration of organic
wastewater compounds by general-use category, Threemile Creek basin, Mobile,
Alabama, 20002003
- Frequencies of detection of selected organic wastewater compounds in water
samples from the the Threemile Creek basin, Mobile, Alabama, and sites
nationwide
- Benthic invertebrate taxa richness at selected sites on Threemile Creek,
Mobile, Alabama, July 2000
- Relative abundance of major benthic invertebrate taxa at selected sites on
Threemile Creek, Mobile, Alabama, July 2000
- Average U.S. Environmental Protection Agency national tolerance values for
benthic invertebrate communities at selected sites on Threemile Creek,
Mobile, Alabama, July 2000
- Shannon-Weaver diversity index values for benthic invertebrate communities
at selected sites on Threemile Creek, Mobile, Alabama, July 2000
- Benthic chlorophyll a concentrations at selected sites in the Threemile Creek
basin, Mobile, Alabama, September 2000
- Benthic chlorophyll a concentrations at selected sites in the Threemile Creek
basin, Mobile, Alabama, 20002003
- Periphyton ash-free dry mass at selected sites in the Threemile Creek basin,
Mobile, Alabama, September 2000
- Periphyton ash-free dry mass at selected sites on Threemile Creek,
Mobile, Alabama, 20002003
- Periphyton taxa richness at selected sites in the Threemile Creek basin,
Mobile, Alabama, 20002002
- Percentage of diatom taxa richness in each saprobic class for periphyton
community samples collected from selected sites in the Threemile Creek
basin, Mobile, Alabama, September 2000
- Percentage of diatom biovolume in each saprobic class for periphyton
community samples collected from selected sites in the Threemile Creek
basin, Mobile, Alabama, September 2000
Tables
- Description of surface-water sites selected for water-quality and biological
sampling in the Threemile Creek basin, Mobile, Alabama, 20002003
- Landuse characteristics in the watersheds of sampling sites in the Threemile
Creek basin, Mobile, Alabama
- Rainfall summary for the Mobile Regional Airport and U.S. Geological Survey
streamgaging station 02471013 (TM-1), Mobile, Alabama
- Population changes (19902000) in the watersheds of sampling sites in the
Threemile Creek basin, Mobile, Alabama
- Number and type of water-quality, benthic invertebrate, and periphyton samples
collected at sites in the Threemile Creek basin, Mobile, Alabama, 20002003
- Saprobic categories of diatoms and corresponding dissolved-oxygen
concentrations and biochemical oxygen demand values
- Results of the Kruskal-Wallis test and the Tukey multiple-comparison test illustrating
statistically significant (p < 0.05) differences for selected water-quality constituents
at sites in the Threemile Creek basin, Mobile, Alabama
- Instantaneous field measurements of water-quality properties at sites in the
Threemile Creek basin, Mobile, Alabama, 20002003, and corresponding
water-quality criteria
- Continuous measurements of water-quality properties at sites in the Threemile
Creek basin, Mobile, Alabama, water years 20002003, and corresponding
water-quality criteria
- Results of multiple-comparison tests on selected nutrients at surface-water
sites in Threemile Creek basin, Mobile, Alabama
- Ambient water-quality criteria recommendations for rivers and streams in
Nutrient Ecoregions IX and XII
- Statistical summary of enterococci, Escherichia coli, and fecal coliform
concentrations at sites in the Threemile Creek basin, Mobile, Alabama, 20002003
- State and Federal standards and criteria for bacteria in surface water
- Exceedance frequencies for fecal-indicator bacteria detected at sites in the
Threemile Creek basin, Mobile, Alabama, 20002003
- Summary of organic wastewater compounds detected in water samples collected
in the Threemile Creek basin, Mobile, Alabama, 20002003
- Detection frequencies and concentrations of selected organic wastewater
compounds in water samples from the Threemile Creek basin, Mobile,
Alabama (20002003) and sites nationwide (19992000)
- Five most abundant benthic invertebrate taxa and the respective percentages of
total abundance for each taxa at selected sites in Threemile Creek, Mobile,
Alabama, July 2000
- North Carolina biotic index values and associated water-quality conditions
- Periphyton chlorophyll a and ash-free dry mass concentrations at selected sites
in the Threemile Creek basin, Mobile, Alabama, 20002003
- Mean total algal cell density and mean percentage of total algal cell density in
major algal groups in samples from selected sites in the Threemile Creek basin,
Mobile, Alabama, 20002002
- Mean total algal biovolume and mean percentage of total algal biovolume in major
algal groups in samples from selected sites in the Threemile Creek basin, Mobile,
Alabama, 20002002
Appendix 1. Quality Control
1-1. Concentrations of nutrients, major ions, and selected organic wastewater
compounds detected in blank samples in the Threemile Creek basin,
Mobile, Alabama, 20002003
Appendix 2. Water-Quality Samples
2-1. Summary of selected constituents at sampling sites in the Threemile Creek basin,
Mobile, Alabama, 20002003
2-2. Concentrations of selected organic wastewater compounds detected at sites
in the Threemile Creek basin, Mobile, Alabama, 20002003
Appendix 3. Benthic Invertebrates and Periphyton
3-1. Benthic invertebrate taxa, percent abundance of each taxon, and total abundance
at selected sites in Threemile Creek, Mobile, Alabama, July 2000
3-2. Periphyton taxa and cell density and biovolume by taxon for samples from selected
sites in the Threemile Creek basin, Mobile, Alabama, 20002002
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