Publications—Scientific Investigations Report 2007-5229

Prepared in cooperation with the Indiana Department of Environmental Management, Division of Water, Assessment Branch


Relations of Principal Components Analysis Site Scores to Algal-Biomass, Habitat, Basin-Characteristics, Nutrient, and Biological-Community Data in the Whitewater River and East Fork White River Basins, Indiana, 2002

By Brian J. Caskey, Jeffrey W. Frey, and B. Scott Lowe

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Abstract

Data were gathered from May through September 2002 at 76 randomly selected sites in the Whitewater River and East Fork White River Basins, Indiana, for algal biomass, habitat, nutrients, and biological communities (fish and invertebrates). Basin characteristics (land use and drainage area) and biolog-ical-community attributes and metric scores were determined for the basin of each sampling site. Yearly Principal Compo-nents Analysis site scores were calculated for algal biomass (periphyton and seston). The yearly Principal Components Analysis site scores for the first axis (PC1) were related using Spearman’s rho to the seasonal algal-biomass, basin-charac-teristics, habitat, seasonal nutrient, and biological-community attribute and metric score data.

The periphyton PC1 site score was not significantly related to the nine habitat or 12 nutrient variables examined. One land-use variable, drainage area, was negatively related to the periphyton PC1. Of the 43 fish-community attributes and metrics examined, the periphyton PC1 was negatively related to one attribute (large-river percent) and one metric score (car-nivore percent metric score). It was positively related to three fish-community attributes (headwater percent, pioneer percent, and simple lithophil percent). The periphyton PC1 was not statistically related to any of the 21 invertebrate-community attributes or metric scores examined.

Of the 12 nutrient variables examined two were nega-tively related to the seston PC1 site score in two seasons: total Kjeldahl nitrogen (July and September), and TP (May and September). There were no statistically significant relations between the seston PC1 and the five basin-characteristics or nine habitat variables examined. Of the 43 fish-community attributes and metrics examined, the seston PC1 was positively related to one attribute (headwater percent) and negatively related to one metric score (large-river percent metric score) . Of the 21 invertebrate-community attributes and metrics exam-ined, the seston PC1 was negatively related to one metric score (number of individuals metric score).

To understand how the choice of sampling sites might have affected the results, an analysis of the drainage area and land use was done. The sites selected in the Whitewater River Basin were generally small drainage basins; compared to Whitewater River Basin sites, the sites selected in the East Fork White River Basin were generally larger drainage basins. Although both basins were dominated by agricultural land use the Whitewater River Basin sites had more land in agriculture than the East Fork White River Basin sites.

The values for nutrients (nitrate, total Kjeldahl nitrogen, total nitrogen, and total phosphorus) and chlorophyll a (per-iphyton and seston) were compared to published U.S. Environmental Protection Agency (USEPA) values for Aggregate Nutrient Ecoregions VI and IX and USEPA Level III Ecore-gions 55 and 71. Several nutrient values were greater than the 25th percentile of published USEPA values. Chlorophyll a (periphyton and seston) values were either greater than the 25thpercentile of published USEPA values or they extended data ranges in the Aggregate Nutrient and Level III Ecore-gions. If the values for the 25th percentile as proposes by the USEPA were adopted as nutrient water-quality criteria, many samples in the Whitewater River and East Fork White River Basins would have exceeded the criteria.

Contents

Abstract

Introduction

Purpose and Scope

Description of the Whitewater River Basin

Description of the East Fork White River Basin

Study Methods

Site Selection and Sampling Strategies

Algal-Biomass, Habitat, Nutrient, and Biological-Community Data-Collection and Processing Methods

Data Analysis

Basin-Characteristics Data

Nutrient Data

Principal Components Analysis

Comparison With U.S. Environmental Protection Agency Data

Relations of the Principal Components Analysis Site Scores to Algal-Biomass, Habitat, Basin-Characteristics, Nutrient, and Biological-Community Data

Drainage-Area and Land-Use Analysis

Comparison of the Data to Ecoregion Nutrient Criteria

Conclusions

Acknowledgments

References

Appendix 1

Figures

  1. Map showing location of the 76 algal-biomass sampling sites in the Whitewater River and East Fork White River Basins, Indiana, May through September, 2002.
  2. Graphs showing the percentages of drainage area and agricultural, forest, and urban land use in the Whitewater River and East Fork White River Basins, Indiana, 2002.

Tables

  1. Location and basin characteristics of the 76 algal-biomass sampling sites in the Whitewater River and East Fork White River Basins, Indiana, May through September, 2002.
  2. Significant Spearman’s rho relations of the yearly periphyton Principal Components Analysis axis 1 site scores (PC1) to algal-biomass, habitat, basin-characteristics, and nutrient parameters and fish- and invertebrate-community attributes and metric scores, 2002.
  3. Significant Spearman’s rho relations of the yearly seston Principal Components Analysis axis 1 site scores (PC1) to algal-biomass, habitat, basin-characteristics, and nutrient parameters and fish- and invertebrate-community attributes and metric scores, 2002.
  4. Basin characteristics of the 76 algal-biomass sampling sites in the Whitewater River and East Fork White River Basins, Indiana, May through September 2002.
  5. Total Kjeldahl nitrogen as N values collected in 2002 from the Indiana Department of Environmental Management/U.S. Geological Survey study and the published U.S. Environmental Protection Agency values for Aggregate Nutrient Ecoregions VI and IX and Level III Ecoregions 55 and 71.
  6. Nitrite plus nitrate as N values collected in 2002 from the Indiana Department of Environmental Management/U.S. Geological Survey study and the published U.S. Environmental Protection Agency values for Aggregate Nutrient Ecoregions VI and IX and Level III Ecoregions 55 and 71.
  7. Total nitrogen as N values collected in 2002 from the Indiana Department of Environmental Management/U.S. Geological Survey study and the published U.S. Environmental Protection Agency values for Aggregate Nutrient Ecoregions VI and IX and Level III Ecoregions 55 and 71.
  8. Total phosphorus as P values collected in 2002 from the Indiana Department of Environmental Management/U.S. Geological Survey study and the published U.S. Environmental Protection Agency values for Aggregate Nutrient Ecoregions VI and IX and Level III Ecoregions 55 and 71.
  9. Mean periphyton chlorophyll a values collected in 2002 from the Indiana Department of Environmental Management/U.S. Geological Survey study and the published U.S. Environmental Protection Agency values for Aggregate Nutrient Ecoregions VI and IX and Level III Ecoregions 55 and 71.
  10. Mean seston chlorophyll a values collected in 2002 from the Indiana Department of Environmental Management/U.S. Geological Survey study and the published U.S. Environmental Protection Agency values for Aggregate Nutrient Ecoregions VI and IX and Level III Ecoregions 55 and 71.

Suggested Citation

Caskey, B.J., Frey, J.W., and Lowe, B. S., 2007, Relations of Principal Component Analysis site scores algal-biomass, habitat, basin-characteristics, nutrient, and biological community data in the Whitewater River and East Fork White River Basin, Indiana, 2002: U.S. Geological Survey Scientific Investigations Report 2007–5229, 31 p.


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