Transcript for Video 1:
These video clips provide visual examples of the physical and swimming abnormalities that we documented using the behavioral checklist during our daily observations.
The fish in these videos are 62-days-post-hatch white sturgeon, 24 hours into a 96-hour exposure. 
This clip is from a control treatment. Notice that some fish occasionally swim upside down and/or rest on the bottom of the test chamber; however, the fish are still swimming with direction.
This fish is displaying a loss of equilibrium, which is an inability to maintain an upright position in the water column, as well as immobilization.
Here are more examples of fish displaying a loss of equilibrium. 
Here is an example of fish displaying an abnormal position in the water column, with most individuals at the bottom of the test chamber.
These fish are displaying some physical abnormalities. Notice the swollen abdomens and spasms. Some fish are also displaying fast, labored respiration as well as changes in pigmentation.

Transcript for Video 2:
This is video footage of 2-days-post-hatch white sturgeon after 96 hours of exposure to copper. This video is from the control treatment. Notice how active the fish are. Also, there are no marked behavioral impairments.
This video is from the 2.66 micrograms per liter of copper treatment, which is very similar to our estimated EC50 of 2.7 micrograms per liter. Notice a reduction in swimming activity compared to the control fish. Also, several fish are displaying a loss of equilibrium, which is the inability to maintain an upright position in the water column. Some fish are also lying immobile on the bottom of the test chamber.
This is video from the 23.6 micrograms per liter of copper treatment. Although some fish appear to be dead, all of these fish moved when prodded. Notice the lack of swimming activity and the apparent loss of equilibrium and immobilization. These are examples of some of the impairments we documented using our behavioral checklist during our daily observations.