By James F. Wilson, Jr., Ernest D. Cobb, and Frederick A. Kilpatrick
Preface
Abstract
Introduction
General description of fluorescence
Fluorescent dyes
Types recommended for tracing
Properties of dyes
Factors that affect fluorescence
Sorption
Biological effects
Spectral characteristics
Fluorometers
General description
Selection of optical components
Lamps
Filters
Sample holders
Photomultiplier
Range control
Preferred systems
Accessory equipment
Temperature-control apparatus
Portable power supplies
Continuous-sampling equipment
Fluorometer operation
General procedure in the laboratory
Preparations
Background readings
Sample testing
Special procedures in the field
Discrete sampling
Flow-through sampling
Troubleshooting
Fluorometer calibration
Concentration units
Preparation of standard solutions
Equipment and supplies
Serial dilution procedure
Testing procedure
Calibration curves
Calibration of the flow-through cuvette
Determination of sample concentration
Aerial photography as a supplement to fluorometry
Selected references
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