Biodegradation of Chlorinated Ethenes at a Karst Site in Middle Tennessee

Contents

Abstract

Introduction

Chlorinated-Ethene Biodegradation Processes

Reductive Dechlorination

Aerobic Cometabolism

Direct Oxidation

Previous Studies Relevant to Biodegradation in Bedrock Aquifers

Study Site

Methods and Procedures

Water-Quality Data Collection

Geochemical Indicators

Chlorinated-Ethene and Degradation Product Data

Microcosm Experiments

Bacteria Identification and Enumeration

Results and Interpretation

Biodegradation of Chlorinated Ethenes in the Shallow Water-Bearing Zone

Biodegradation of Chlorinated Ethenes in the Karst Aquifer

Multiple Lines of Evidence from Well 12D

Multiple Lines of Evidence from Well 1D

Multiple Lines of Evidence from Well 3D

Multiple Lines of Evidence from Well 2D

Data Collected from Other Deep Wells

Lessons Learned

Summary and Conclusions

References

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