Total Carbon	Organic Carbon
Acetanilide (Conditioner) - C: 71.09% H: 6.71% N: 10.36%	Acetanilide (Conditioner) - C: 71.09% H: 6.71% N: 10.36%
Acetanilide (K-Factor) - C: 13.41% H: 27.45% N: 4.73%	Acetanilide (K-Factor) - C: 13.38% H: 45.36% N: 4.68%
Marine Sediment (MESS-2) - C: 2.92% H: 0.71% N: 0.18%	Marine Sediment (MESS-2) - C: 2.91% H: 0.32% N: 0.14%

Marine Sediment (MESS-1) - C: 2.92%

Marine Sediment (MESS-1) - C: 2.91%

MESS-2 standard is no longer available from the manufacturer -- see National Research Council of Canada website for details.

The Acetanilide standard is multi-purpose. It can be run as a Conditioner sample at the very beginning
of a run to make sure the machine is running correctly. Acetanilide has known values for carbon,
hydrogen, and nitrogen so when it is weighed (1-3mg) and run as a Conditioner sample, the values should be
very close to the known values given above. Conditioner samples are never acidified. Acetanilide can also be
run as a K-Factor standard to help calibrate the machine. When it is weighed (1-3mg) and run as a K-Factor,
whether it be for Total or Organic Carbon, the average values should be very close to those given above.
If either the Conditioner or K-Factor values differ greatly (+/- 10 %) from those given above, call the Perkin
Elmer Service Representative.

Standard	Composition	Purpose
Conditioner	1-3mg Acetanilide	Initial analysis of known percentages to ensure proper machine function
K-Factor	1-3mg Acetanilide	Analysis of average known percentages used to calibrate machine
MESS-2	10mg Marine Sediment	Analysis of average known percentages used to calibrate machine
Blank	Clean & Empty Capsule	Used to assure instrument integrity

*For Organic Carbon, follow the sequence through #4, then use acidified standards for 5-7, then start running acidified samples.

K-factors should reproduce from the mean value to within these limits:

Carbon	Hydrogen	Nitrogen
+/- 0.15	+/- 3.75	+/- 0.16

*For Organic Standards:
Mess-2 - weigh out 10mg of standard and acidify with 150µL Sulfurous Acid
Blanks - acidify capsules only
K-Factors - acidify capsule only, then add 1-3mg Acetanilide to acidified capsule
Conditioner - weigh 1-3mg of standard and run (no acidification)
**See Excel Spreadsheet for Run Sequence:
Total Carbon Sequence
Organic Carbon Sequence