The seasonal use of an Ozark spring branch by fishes was evaluated from January 1993 to January 1995. Temperatures in the spring branch were fairly constant, at 13.5 ± 1°C, and usually exceeded the temperature of a receiving stream during the November–March coldwater period. Black redhorse Moxostoma duquesnei, smallmouth bass Micropterus dolomieu, shadow bass Ambloplites ariommus, and longear sunfish Lepomis megalotis showed a temporal pattern of use in the spring branch. The lowest use was during the April–October warmwater period, and the greatest use was during the coldwater period. During cold periods, biomass of these four species in the spring branch was significantly greater and fish size significantly larger than in similar habitats in the adjoining stream. The weight per unit length of fish using the spring branch during cold periods was also significantly greater. Shadow bass and longear sunfish had significantly greater relative stomach contents than conspecifics in equivalent habitats from the adjoining stream, but had similar relative numbers of eggs. Large concentrations of adult fish used spring branches during the coldwater period, and these fish may be more vulnerable to angling than conspecifics in similar habitats in adjoining streams dominated by surface water. Thus, we suggest that stream managers consider the usefulness of fish refuges in spring branches and adjacent thermally influenced areas.