Liquid chromatographic determination of florfenicol in the plasma of multiple species of fish
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
By: C. Vue, Larry J. Schmidt, Guy R. Stehly, and William H. Gingerich
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Abstract
A simple method was developed for determining florfenicol concentration in a small volume (250 μl) of plasma from five phylogenetically diverse species of freshwater fish. Florfenicol was isolated from the plasma matrix through C18 solid-phase extraction and quantified by reversed-phase high-performance liquid chromatography with UV detection. The accuracy (84–104%), precision (%RSD⩽8), and sensitivity (quantitation limit <30 ng/ml) of the method indicate its usefulness for conducting pharmacokinetic studies on a variety of freshwater fish.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Liquid chromatographic determination of florfenicol in the plasma of multiple species of fish |
| Series title | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| DOI | 10.1016/S1570-0232(02)00441-5 |
| Volume | 780 |
| Issue | 1 |
| Year Published | 2002 |
| Language | English |
| Publisher | Elsevier |
| Contributing office(s) | Upper Midwest Environmental Sciences Center |
| Description | 7 p. |
| First page | 111 |
| Last page | 117 |