Screening fungicides for use in fish culture: Evaluation of the agar plug transfer, cellophane transfer, and agar dilution methods
Progressive Fish-Culturist
By: Tom A. Bailey
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Abstract
The reliability, reproducibility, and usefulness of three screening methods -- the cellophane transfer, the agar plug transfer, and the agar dilution -- to screen aquatic fungicides were evaluated. Achlya flagellata and Saprolegnia hypogyna were exposed to 1, 10, and 100 mg/L of malachite green to test each method. The cellophane transfer and agar plug transfer techniques had similar reliability and reproducibility in rating fungicidal activity, and were both superior to the agar dilution technique. The agar plug transfer and agar dilution techniques adequately projected in vivo activity of malachite green, but the cellophane transfer technique overestimated its activity. Overall, the agar plug transfer technique most accurately rated the activity of malachite green and was the easiest test to perform. It therefore appears to be the method of choice for testing aquatic fungicides.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Screening fungicides for use in fish culture: Evaluation of the agar plug transfer, cellophane transfer, and agar dilution methods |
| Series title | Progressive Fish-Culturist |
| DOI | 10.1577/1548-8659(1983)45[24:SFFUIF]2.0.CO;2 |
| Volume | 45 |
| Issue | 1 |
| Year Published | 1983 |
| Language | English |
| Publisher | Oxford Academic |
| Contributing office(s) | Upper Midwest Environmental Sciences Center |
| Description | 4 p. |
| First page | 24 |
| Last page | 27 |