Rapid method for measuring rotenone in water at piscicidal concentrations
Links
- More information: Publisher Index Page (via DOI)
- Download citation as: RIS | Dublin Core
Abstract
A high‐performance liquid chromatography (HPLC) procedure that is rapid, specific, and sensitive (limit of detection <0.005 mg/liter) was developed for monitoring application and degradation rates of rotenone. For analysis, a water sample is buffered to pH 5 and injected through a Sep Pak(R) C18 disposable cartridge. The cartridge adsorbs and retains the rotenone which then can be eluted quantitatively from the cartridge with a small volume of methanol. This step effectively concentrates the sample and provides sample cleanup. The methanol extract is analyzed directly by HPLC on an MCH 10 reverse‐phase column; methanol: Water (75:25, volume : Volume) is the mobile phase and flow rate is 1.5 ml/minute. The rotenone is detected by ultraviolet spectrophotometry at a wavelength of 295 nm.
Suggested Citation
Dawson, V.K., Harman, P., Schultz, D., and Allen, J.L., 1983, Rapid method for measuring rotenone in water at piscicidal concentrations: Transactions of the American Fisheries Society, v. 112, no. 5, p. 725-727, https://doi.org/10.1577/1548-8659(1983)112<725:RMFMRI>2.0.CO;2.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Rapid method for measuring rotenone in water at piscicidal concentrations |
| Series title | Transactions of the American Fisheries Society |
| DOI | 10.1577/1548-8659(1983)112<725:RMFMRI>2.0.CO;2 |
| Volume | 112 |
| Issue | 5 |
| Year Published | 1983 |
| Language | English |
| Publisher | American Fisheries Society |
| Contributing office(s) | Upper Midwest Environmental Sciences Center |
| Description | 3 p. |
| First page | 725 |
| Last page | 727 |