Gas-liquid chromatographic determination of Bayer 73 in fish, aquatic invertebrates, mud, and water

Journal of the Association of Official Analytical Chemists
By: , and 

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Abstract

A gas-liquid chromatographic (GLC) method is described for determining residues of Bayer 73 (2-aminoethanol salt of 2’,5-dichloro-4’-nitrosalicylanilide) in fish muscle, aquatic invertebrates, mud, and water by analyzing for 2-chloro-4-nitroaniline (CNA), a hydrolysis product of Bayer 73. Bayer 73 residues are extracted from fish muscle tissue, invertebrates, and mud with acetone-formic acid (98+2), and partitioned from water samples with chloroform. After sample cleanup by solvent and acid-base partitioning, the concentrated extract is hydrolyzed with 2N NaOH and H2O2 for 10 min at 95°C. The CNA is then partitioned into hexane-ethyl ether (7+3) and determined by electron capture GLC. Average recoveries were 88% for fish, 82% for invertebrates, 82% for mud, and 98% for water at 3 or more fortification levels.

Publication type Article
Publication Subtype Journal Article
Title Gas-liquid chromatographic determination of Bayer 73 in fish, aquatic invertebrates, mud, and water
Series title Journal of the Association of Official Analytical Chemists
DOI 10.1093/jaoac/62.5.1141
Volume 62
Issue 5
Publication Date January 22, 2020
Year Published 1979
Language English
Publisher Oxford Academic
Contributing office(s) Upper Midwest Environmental Sciences Center
Description 5 p.
First page 1141
Last page 1145
Additional publication details