Induction of sporulation and the influence of time, temperature, and inoculum size on growth in two species of aquatic fungi (Saprolegniales)
Journal of the Elisha Mitchell Scientific Society
By: T.A. Bailey, K. Bradford, and C.E. Bland
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Abstract
Because the infective stage of most mycoses of aquatic organisms is the zoospore, we attempted to establish optimum conditions under which zoospores could be produced for use in antifungal testing. Optimum sporulation time, incubation time, inoculum size, and growth temperature were determined for each oftwo saprolegniaceous fungi, Achlya flagellata Coker and Saprolegnia hypogyna (Pringsheim) de Bary. Both species produced the largest number of zoospores after 18 hours (51.7 spores/ml for A. jlagellata and 848.0 spores/ml for S. hypogyna), and yielded maximum growth after 48 hours at 22 'C. The recommended test inoculum size for S. hypogyna (5,600 spores/ml was nearly three times that for A. flagellata (2,000 spores/ml),
Suggested Citation
Bailey, T., Bradford, K., Bland, C., 1990, Induction of sporulation and the influence of time, temperature, and inoculum size on growth in two species of aquatic fungi (Saprolegniales): Journal of the Elisha Mitchell Scientific Society, v. 106, no. 3, p. 57-63.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Induction of sporulation and the influence of time, temperature, and inoculum size on growth in two species of aquatic fungi (Saprolegniales) |
| Series title | Journal of the Elisha Mitchell Scientific Society |
| Volume | 106 |
| Issue | 3 |
| Year Published | 1990 |
| Language | English |
| Contributing office(s) | Upper Midwest Environmental Sciences Center |
| Description | pp. 57-63 |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Journal of the Elisha Mitchell Scientific Society |
| First page | 57 |
| Last page | 63 |