Myxosoma cerebralis: Fluorescent antibody techniques for antigen recognition
Journal of the Fisheries Research Board of Canada
By: M.E. Markiw and K. Wolf
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Abstract
Rabbits were immunized with antigens extracted from mature spores or prespore stages of Myxosoma cerebralis, and the resulting antisera and their globulins were used in direct and indirect fluorescent antibody techniques. Both kinds of antisera reacted with homologous spores and with stages of the organism that precede spores. When tested for specificity against spores of 12 other myxosporidans the direct fluorescent antibody technique showed cross-reactivity with only one other and that was a species of Myxosoma. The indirect fluorescent antibody technique showed some reactions across generic lines. The antisera have application in studies of the parasite's life cycle and in diagnostics.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Myxosoma cerebralis: Fluorescent antibody techniques for antigen recognition |
| Series title | Journal of the Fisheries Research Board of Canada |
| DOI | 10.1139/f78-133 |
| Volume | 35 |
| Issue | 6 |
| Year Published | 1978 |
| Language | English |
| Publisher | Canadian Science Publishing |
| Contributing office(s) | Leetown Science Center |
| Description | 5 p. |
| First page | 828 |
| Last page | 832 |