Detection of Vibrio anguillarum antigen by the dot blot assay
Journal of Wildlife Diseases
By: R.C. Cipriano, J.B. Pyle, C.E. Starliper, and S. W. Pyle
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Abstract
The dot blot assay, modified and adapted for detection of antigens from Vibrio anguillarum in fish tissues, was specific for V. anguillarum and did not react with antigens of V. ordalii, Pseudomonas sp., or Yersinia ruckeri. The blot assay enabled detection of as little as 2.3 ng of a mixture of protein antigens obtained from cell-free extracts of V. anguillarum; it was about 100 times more sensitive than either the indirect fluorescent antibody technique or bacterial isolation for detecting V. anguillarum in fish tissues.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Detection of Vibrio anguillarum antigen by the dot blot assay |
| Series title | Journal of Wildlife Diseases |
| DOI | 10.7589/0090-3558-21.3.211 |
| Volume | 21 |
| Issue | 3 |
| Year Published | 1985 |
| Language | English |
| Publisher | Wildlife Disease Association |
| Contributing office(s) | Leetown Science Center |
| Description | 8 p. |
| First page | 211 |
| Last page | 218 |
| Google Analytic Metrics | Metrics page |