Herpesvirus salmonis: Characterization of a new pathogen of rainbow trout
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Abstract
A new agent, provisionally designated Herpesvirus salmonis, was isolated from post-spawning rainbow trout (Salmo gairdneri) and studied primarily in the RTG-2 rainbow trout cell line. Infection of RTG-2 cells resulted in the formation of syncytia and Cowdry type A intranuclear inclusions. Replication occurred regularly at 5 and 10°C, but was inconsistent at 15°C, largely inhibited at 0°C, and completely inhibited at 20°C or higher. The virus was acid, heat, ether, and chloroform labile, but stable to freezing and thawing. It did not hemagglutinate. Viral DNA had a buoyant density of 1.709 g/cm3 and a guanine-cytosine value of 50%. Hexagonal nucleocapsids had a diameter of 90 nm and were first seen in nuclei at 36 h. Enveloped forms measured about 150 nm and occurred both cytoplasmically and extracellularly. At 10°C, a one-step growth culture required about 96 h; cell-associated virus peaked at about 105 PFU/ml and exceeded released virus by a factor of about 10.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Herpesvirus salmonis: Characterization of a new pathogen of rainbow trout |
| Series title | Journal of Virology |
| DOI | 10.1128/jvi.27.3.659-666.1978 |
| Volume | 27 |
| Issue | 3 |
| Year Published | 1978 |
| Language | English |
| Publisher | American Society for Microbiology |
| Contributing office(s) | Leetown Science Center |
| Description | 8 p. |
| First page | 659 |
| Last page | 666 |