Production and characterization of monoclonal antibody against infectious hematopoietic necrosis virus
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Abstract
A hybridoma cell line that continually secretes antibody against infectious hematopoietic necrosis virus was developed and designated M-IHNV-W1. Antibodies in culture fluids were concentrated and purified by Protein A Sepharose CL-4B affinity chromatography. The immunoglobulin was isotyped as IgG 2b with a kappa light chain. The IgG was specific for infectious hematopoietic necrosis virus and had binding activity but low neutralizing activity. Little cross-reactivity occurred with viral hemorrhagic septicemia or infectious pancreatic necrosis viruses.
Suggested Citation
Schultz, C.L., Lidgerding, B.C., McAllister, P.E., Hetrick, F.M., 1985, Production and characterization of monoclonal antibody against infectious hematopoietic necrosis virus: Fish Pathology, v. 20, no. 2-3, p. 339-341, https://doi.org/10.3147/jsfp.20.339.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Production and characterization of monoclonal antibody against infectious hematopoietic necrosis virus |
| Series title | Fish Pathology |
| DOI | 10.3147/jsfp.20.339 |
| Volume | 20 |
| Issue | 2-3 |
| Year Published | 1985 |
| Language | English |
| Publisher | Japanese Society of Fish Pathology |
| Contributing office(s) | Leetown Science Center |
| Description | 3 p. |
| First page | 339 |
| Last page | 341 |