Production and characterization of monoclonal antibody against infectious hematopoietic necrosis virus
Fish Pathology
By: C. L. Schultz, B. C. Lidgerding, P. E. McAllister, and F. M. Hetrick
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Abstract
A hybridoma cell line that continually secretes antibody against infectious hematopoietic necrosis virus was developed and designated M-IHNV-W1. Antibodies in culture fluids were concentrated and purified by Protein A Sepharose CL-4B affinity chromatography. The immunoglobulin was isotyped as IgG 2b with a kappa light chain. The IgG was specific for infectious hematopoietic necrosis virus and had binding activity but low neutralizing activity. Little cross-reactivity occurred with viral hemorrhagic septicemia or infectious pancreatic necrosis viruses.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Production and characterization of monoclonal antibody against infectious hematopoietic necrosis virus |
| Series title | Fish Pathology |
| DOI | 10.3147/jsfp.20.339 |
| Volume | 20 |
| Issue | 2-3 |
| Year Published | 1985 |
| Language | English |
| Publisher | Japanese Society of Fish Pathology |
| Contributing office(s) | Leetown Science Center |
| Description | 3 p. |
| First page | 339 |
| Last page | 341 |