Effects of prolacton chloride cells opercular membrane of seawater-adapted tilapia
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Abstract
Effects of prolactin on morphology and numbers of chloride cells in the opercular membrane of seawater-adapted tilapia (Oreochromis mossambicus) have been examined. Following five daily injections of ovine prolactin at a dose of 10 μg · g body wt−1, blood samples were taken and opercular membranes were removed and stained with a fluorescent mitochondrial dye (dimethylaminostyrylethylpyridiniumiodine), a fluorescent derivative of ouabain (anthroylouabain), and a histological stain specific for the extensive tubular system of chloride cells (zinc-osmium-iodine). Mean plasma osmolarity and sodium increased 23–24% following prolactin injection. An increase in the relative frequency of chloride cells between 20 and 180 μm2 in cross-sectional area and a decrease in the relative frequency of chloride cells greater than 180 μm2 were observed following prolactin injections. Average cell size decreased 46–70% and cell height decreased 26–38% following prolactin injections. There was no significant change in cell density. Anthroylouabain staining was observed in both prolactin- and saline-injected fish, and no significant effect on Na+,K+-adenosinetri-phosphatase activity was seen in either opercular membrane or gill tissue. The results demonstrate an effect of prolactin on chloride cell size and provide a morphological correlate for decreased secretory activity of chloride cells following prolactin injections.
Suggested Citation
Herndon, T., McCormick, S., and Bern, H.A., 1991, Effects of prolacton chloride cells opercular membrane of seawater-adapted tilapia: General and Comparative Endocrinology, v. 83, no. 2, p. 283-289, https://doi.org/10.1016/0016-6480(91)90032-2.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Effects of prolacton chloride cells opercular membrane of seawater-adapted tilapia |
| Series title | General and Comparative Endocrinology |
| DOI | 10.1016/0016-6480(91)90032-2 |
| Volume | 83 |
| Issue | 2 |
| Year Published | 1991 |
| Language | English |
| Publisher | Elsevier |
| Contributing office(s) | Leetown Science Center |
| Description | 7 p. |
| First page | 283 |
| Last page | 289 |