Predation on recently released larval American shad Alosa sapidissima was quantified in the Juniata River, Pennsylvania, on 10 occasions during 1991 and 1992. Of the four sites examined (the stocking site and 100, 200, and 350 m downstream) predation on shad larvae was highest at the stocking site; 44% of the total estimated losses occurred within this 30-m zone. Percentage predation mortality was weakly and inversely related to the number of shad larvae released, and it ranged from 0 to 2.2% among sites. Overall percent mortality of larvae within the first 2 h of release was about 5% (range, 2–10%). Major predators included juvenile smallmouth bass Micropterus dolomieu, the spotfin shiner Cyprinella spiloptera, and the mimic shiner Notropis volucellus. Predation by these species varied among sites. Percent mortality of American shad larvae after nocturnal releases (0.17%) was significantly lower than after diurnal releases (1.18%). For most predators, the length of shad eaten increased with predator length. However, among predators of similar size, there were significant differences in the length of shad consumed. Estimated losses to predation were about equally divided among small (<50 mm, 30%) medium (50–99 mm, 37%), and large (>99 mm, 33%) predators. Because 30% of the estimated number of shad larvae lost to predation were eaten by predators less than 50 mm long, releasing slightly larger shad might reduce predation.