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Incidence of trypanosomes in the Canada goose as revealed by bone marrow culture

Journal of Parasitology
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Abstract

1. Techniques are described for the cultural isolation of trypanosomes from avian bone marrow obtained from living birds or at autopsy. A new medium SNB-9 (saline-neopeptone-blood) is described. In addition to being a good medium for growing avian trypanosomes, it is excellent for growing trypanosomes of amphibians and mammals. 2. Evidence is presented demonstrating the superiority of (a) cultures over stained smears for detecting the presence of trypanosomes in the Canada goose, and (b) bone marrow over heart blood of this species as a source of trypanosomes for culture. 3. In April 1952, from cultures of bone marrow collected at autopsy it was demonstrated that trypanosome infection occurred in 33 (40.2%) of 82 Canada geese from the Pea Island National Wildlife Refuge. On February 17, 1953, cultures of bone marrow obtained from living birds revealed presence of trypanosomes in 12 (20.7%) of 58 geese from the same refuge. On February 26, 1953, by employing the latter method, 9 (20.4%) of 44 geese from Blackwater National Wildlife Refuge were shown to harbor the parasites. In another survey ninety-two geese from seven national wildlife refuges subjected to the biopsy technique showed evidence of infection in 13 (14.1 %) birds and indicated that trypanosome infection is widely distributed in this host.
Publication type Article
Publication Subtype Journal Article
Title Incidence of trypanosomes in the Canada goose as revealed by bone marrow culture
Series title Journal of Parasitology
Volume 40
Issue 2
Year Published 1954
Language English
Contributing office(s) Patuxent Wildlife Research Center
Description 195-202
Larger Work Type Article
Larger Work Subtype Journal Article
Larger Work Title Journal of Parasitology
First page 195
Last page 202
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