Sperm quality assessments for endangered razorback suckers Xyrauchen Texanus
Reproduction
Links
- More information: Publisher Index Page (via DOI)
- Open Access Version: Publisher Index Page
- Download citation as: RIS | Dublin Core
Abstract
Flow cytometry (FCM) and computer-assisted sperm motion analysis (CASA) methods were developed and validated for use with endangered razorback suckers Xyrauchen texanus collected (n=64) during the 2006 spawning season. Sperm motility could be activated within osmolality ranges noted during milt collections (here 167–343 mOsm/kg). We hypothesized that sperm quality of milt collected into isoosmotic (302 mOsm/kg) or hyperosmotic (500 mOsm/kg) Hanks' balanced salt solution would not differ. Pre-freeze viabilities were similar between osmolalities (79%±6 (S.E.M.) and 76%±7); however, post-thaw values were greater in hyperosmotic buffer (27%±3 and 12%±2; P=0.0065), as was mitochondrial membrane potential (33%±4 and 13%±2; P=0.0048). Visual estimates of pre-freeze motility correlated with total (r=0.7589; range 23–82%) and progressive motility (r=0.7449) by CASA and were associated with greater viability (r=0.5985; P<0.0001). Count (FCM) was negatively correlated with post-thaw viability (r=-0.83; P=0.0116) and mitochondrial function (r=-0.91; P=0.0016). By FCM-based assessments of DNA integrity, whereby increased fluorochrome binding indicated more fragmentation, higher levels were negatively correlated with count (r=-0.77; P<0.0001) and pre-freeze viabilities (r=-0.66; P=0.0004). Fragmentation was higher in isotonic buffer (P=0.0234). To increase reproductive capacity of natural populations, the strategy and protocols developed can serve as a template for use with other imperiled fish species, biomonitoring, and genome banking.
Suggested Citation
Jenkins, J.A., Eilts, B.E., Guitreau, A.M., Figiel, C.R., Draugelis-Dale, R.O., and Tiersch, T.R., 2011, Sperm quality assessments for endangered razorback suckers Xyrauchen texanus: Reproduction, v. 141, no. 1, p. 55-65, https://doi.org/10.1530/REP-10-0153.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Sperm quality assessments for endangered razorback suckers Xyrauchen texanus |
| Series title | Reproduction |
| DOI | 10.1530/REP-10-0153 |
| Volume | 141 |
| Issue | 1 |
| Year Published | 2011 |
| Language | English |
| Publisher | Society for Reproduction and Fertility |
| Contributing office(s) | National Wetlands Research Center |
| Description | 11 p. |
| First page | 55 |
| Last page | 65 |
| Time Range Start | 2006-01-01 |
| Time Range End | 2006-12-31 |