A noninvasive, direct real-time PCR method for sex determination in multiple avian species
Molecular Ecology Resources
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Abstract
Polymerase chain reaction (PCR)-based methods to determine the sex of birds are well established and have seen few modifications since they were first introduced in the 1990s. Although these methods allowed for sex determination in species that were previously difficult to analyse, they were not conducive to high-throughput analysis because of the laboriousness of DNA extraction and gel electrophoresis. We developed a high-throughput real-time PCR-based method for analysis of sex in birds, which uses noninvasive sample collection and avoids DNA extraction and gel electrophoresis.
Suggested Citation
Brubaker, J.L., Karouna-Renier, N., Chen, Y., Jenko, K., Sprague, D.T., Henry, P.F., 2011, A noninvasive, direct real-time PCR method for sex determination in multiple avian species: Molecular Ecology Resources, v. 11, no. 2, p. 415-417, https://doi.org/10.1111/j.1755-0998.2010.02951.x.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | A noninvasive, direct real-time PCR method for sex determination in multiple avian species |
| Series title | Molecular Ecology Resources |
| DOI | 10.1111/j.1755-0998.2010.02951.x |
| Volume | 11 |
| Issue | 2 |
| Publication Date | December 12, 2010 |
| Year Published | 2011 |
| Language | English |
| Publisher | Wiley |
| Contributing office(s) | Patuxent Wildlife Research Center, Contaminant Biology Program |
| Description | 3 p. |
| First page | 415 |
| Last page | 417 |