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Abstract
Investigations into cellular and molecular characteristics of male gametes obtained from fish in natural ecosystems require careful sample handling and shipping in order to minimize artifacts. Maintaining sample integrity engenders confident assessments of ecosystem health, whereby animal condition is often reflected by gamete biomarkers - indicators that respond in measurable ways to changes. A number of our investigations have addressed the hypothesis that biomarkers from fish along a pollution gradient are reflective of site location. Species biology and the selected biological endpoints direct choice of parameters such as: temperature, buffer osmolality, time in transit, fixation, cryoprotectants, protease inhibition, and antibiotic inclusion in extender. This paper will highlight case studies, and outline parameters and thoughts on approaches for use by field and laboratory researchers.
| Publication type | Conference Paper |
|---|---|
| Publication Subtype | Conference Paper |
| Title | Review of concepts useful for maintaining quality of male reproductive field samples for laboratory study |
| Year Published | 2011 |
| Language | English |
| Publisher | Diamond Congress Ltd. |
| Publisher location | Budapest, Hungary |
| Contributing office(s) | National Wetlands Research Center |
| Description | 3 p. |
| Larger Work Type | Book |
| Larger Work Subtype | Conference publication |
| Larger Work Title | 3rd International Workshop on the Biology of Fish Gametes, Budapest, Hungary, 7-9 September 2011 |
| First page | 75 |
| Last page | 77 |
| Google Analytic Metrics | Metrics page |