Post-column reaction for simultaneous analysis of chromatic and leuco forms of malachite green and crystal violet by high-performance liquid chromatography with photometric detection
Journal of Chromatography
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Abstract
The chromatic and leuco forms of malachite green and crystal violet were readily separated and detected by a sensitive and selective high-performance liquid chromatographic procedure. The chromatic and leuco forms of the dyes were separated within 11 min on a C18 column with a mobile phase of 0.05 M sodium acetate and 0.05 M acetic acid in water (19%) and methanol (81%). A reaction chamber, containing 10% PbO2 in Celite 545, was placed between the column and the spectrophotometric detector to oxidize the leuco forms of the dyes to their chromatic forms. Chromatic and leuco malachite green were quantified by their absorbance at 618 nm; and chromatic and leuco Crystal Violet by their absorbance at 588 nm. Detection limits for chromatic and leuco forms of both dyes ranged from 0.12 to 0.28 ng. A linear range of 1 to 100 ng was established for both forms of the dyes.
Suggested Citation
Allen, J.L., Meinertz, J., 1991, Post-column reaction for simultaneous analysis of chromatic and leuco forms of malachite green and crystal violet by high-performance liquid chromatography with photometric detection: Journal of Chromatography, v. 536, no. 1-2, p. 217-222, https://doi.org/10.1016/S0021-9673(01)89252-9.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Post-column reaction for simultaneous analysis of chromatic and leuco forms of malachite green and crystal violet by high-performance liquid chromatography with photometric detection |
| Series title | Journal of Chromatography |
| DOI | 10.1016/S0021-9673(01)89252-9 |
| Volume | 536 |
| Issue | 1-2 |
| Year Published | 1991 |
| Language | English |
| Publisher | Elsevier |
| Publisher location | Amsterdam, Netherlands |
| Contributing office(s) | Upper Midwest Environmental Sciences Center |
| Description | 6 p. |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Journal of Chromatography |
| First page | 217 |
| Last page | 222 |