Liquid chromatographic determination of chloramine-T and its primary degradation product, p-toluenesulfonamide, in water
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Abstract
N-sodium-N-chloro-ρ-toiuenesulfonamide (chloramine-T) effectively controls bacterial gill disease (BGD) in cultured fishes. BGD, a common disease of hatchery-reared salmonids, causes more fish losses than any other disease among these species. This study describes a liquid chromatographic (LC) method that is capable of direct, simultaneous analysis of chloramine-T and its primary degradation product, ρ-toluenesulfonamide (ρ-TSA), in water. The procedure involves reversed-phase (C18) LC analysis with ion suppression, using 0.01 M phosphate buffer at pH 3. The mobile phase is phosphate buffer-acetonitrile (60 + 40) at 1 mL/min. Both chemicals can be detected with a UV spectrophotometer at 229 nm; the method is linear up to 40 mg chloramine-T or ρ-TSA/L. Mean recoveries were 96.4 ± 6.1% for water samples fortified with 0.03 mg chloramine-T/L and 95.3 ± 4.6% for water samples fortified with 0.005 mg ρ-TSA/L. Limits of detection without sample enrichment for chloramine-T and ρ-TSA are 0.01 mg/L and 0.001 mg/L, respectively.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Liquid chromatographic determination of chloramine-T and its primary degradation product, p-toluenesulfonamide, in water |
| Series title | Journal of AOAC International |
| DOI | 10.1093/jaoac/80.2.316 |
| Volume | 80 |
| Issue | 2 |
| Year Published | 1997 |
| Language | English |
| Publisher | Oxford Academic |
| Contributing office(s) | Upper Midwest Environmental Sciences Center |
| Description | 3 p. |
| First page | 316 |
| Last page | 318 |
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