Analysis of antimycin A by reversed-phase liquid chromatography/nuclear magnetic-resonance spectrometry
Analytical Chemistry
By: Steven T. K. Ha, Charles L. Wilkins, and Sharon L. Abidi
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Abstract
A mixture of closely related streptomyces fermentation products, antimycin A, Is separated, and the components are identified by using reversed-phase high-performance liquid chromatography with directly linked 400-MHz proton nuclear magnetic resonance detection. Analyses of mixtures of three amino acids, alanine, glycine, and valine, are used to determine optimal measurement conditions. Sensitivity increases of as much as a factor of 3 are achieved, at the expense of some loss in chromatographic resolution, by use of an 80-μL NMR cell, Instead of a smaller 14-μL cell. Analysis of the antimycin A mixture, using the optimal analytical high performance liquid chromatography/nuclear magnetic resonance conditions, reveals it to consist of at least 10 closely related components.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Analysis of antimycin A by reversed-phase liquid chromatography/nuclear magnetic-resonance spectrometry |
| Series title | Analytical Chemistry |
| DOI | 10.1021/ac00180a005 |
| Volume | 61 |
| Issue | 5 |
| Year Published | 1989 |
| Language | English |
| Publisher | ACS Publications |
| Publisher location | Washington, D.C. |
| Contributing office(s) | Upper Midwest Environmental Sciences Center |
| Description | 5 p. |
| First page | 404 |
| Last page | 408 |
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