Low-temperature incubation using a water supply
Applied Microbiology
By: K. Wolf and M. C. Quimby
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Abstract
Cell and tissue culture has been concerned primarily with homiothermic vertebrate cells which require incubation at about 37 C, and there is a great variety of incubators designed to maintain temperatures which are usually above ambient. The culture of poikilothermic vertebrate cells--and invertebrate, plant, and some microbial cells--can often be carried out at ambient temperatures, but for some work cooler conditions must be provided. Variety among the so-called low-temperature incubators is somewhat restricted; there are no small units, and all require a power source to maintain temperatures below ambient. We have used a gravity-fed water supply for 5 years to provide trouble-free, constant, low-temperature incubation of stock cultures of fish and amphibian cells. Though it is but a small part of our low-temperature incubator capacity, it has no power requirements and it provides maximal protection against temperature rises which could be lethal to some of the cell lines. Though the system has limitations, there is a considerable likelihood that the domestic water supply in other laboratories can also be used to provide low-temperature incubation.
Suggested Citation
Wolf, K., Quimby, M.C., 1967, Low-temperature incubation using a water supply: Applied Microbiology, v. 15, no. 6, p. 1501-1501, https://doi.org/10.1128/am.15.6.1501-1501.1967.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Low-temperature incubation using a water supply |
| Series title | Applied Microbiology |
| DOI | 10.1128/am.15.6.1501-1501.1967 |
| Volume | 15 |
| Issue | 6 |
| Year Published | 1967 |
| Language | English |
| Publisher | American Society for Microbiology |
| Publisher location | Washington, D.C. |
| Contributing office(s) | Leetown Science Center |
| Description | 1 p. |
| First page | 1501 |
| Last page | 1501 |